【摘要】：物種分子系統(tǒng)關(guān)系是進(jìn)化生物學(xué)領(lǐng)域最基本也是最重要的問(wèn)題之一。本研究通過(guò)測(cè)序分析了來(lái)自廣西等地90個(gè)變色樹(shù)蜥Calotes versicolor的ND2-tRNA基因片段,分析了不同地理種群的單倍型、種群間的遺傳分化程度(F_(st))和歷史動(dòng)態(tài),構(gòu)建了單倍型之間的分子系統(tǒng)關(guān)系。結(jié)果發(fā)現(xiàn),所研究的地理種群共檢測(cè)到45個(gè)單倍型,總體呈現(xiàn)較高的單倍型多樣性(0.941 8)和較低的核苷酸多樣性(0.005 5),其中有6個(gè)地理種群的個(gè)體共享1個(gè)單倍型。大部分種群間F_(st)值較高且差異顯著,遺傳分化程度較高。分子方差分析結(jié)果表明,遺傳變異主要來(lái)自種群內(nèi)的遺傳差異。系統(tǒng)進(jìn)化分析結(jié)果表明,所研究的地理種群并沒(méi)有出現(xiàn)明顯的遺傳分支且在地理上沒(méi)有嚴(yán)格的分布范圍和分化,這可能是由于種群間存在漸滲雜交或不完全的譜系篩選。網(wǎng)絡(luò)關(guān)系圖也顯示各采樣種群間不存在明顯的譜系結(jié)構(gòu)。種群歷史動(dòng)態(tài)分析表明,各地理種群不存在擴(kuò)張現(xiàn)象。把所有地理種群合并在一起分析表明,貝葉斯輪廓圖分析與堿基錯(cuò)配分析方法均檢測(cè)到種群在歷史上發(fā)生過(guò)快速擴(kuò)張,約0.05 Ma前存在種群擴(kuò)張現(xiàn)象。
[Abstract]:Species molecular system relationship is one of the most basic and important problems in the field of evolutionary biology. In this study, the ND2-tRNA gene fragments of Calotes versicolor from 90 species in Guangxi and other regions were sequenced. The haplotypes of different geographical populations and the degree of genetic differentiation (F _ (st) and historical dynamics) among populations were analyzed. The molecular phylogenetic relationship among haplotypes was constructed. The results showed that a total of 45 haplotypes were detected in the geographical populations, showing a higher haplotype diversity (0.941 8) and a lower nucleotide diversity (0.005 5), and that the population was characterized by high haplotype diversity (0.941 8) and low nucleotide diversity (0.005 5). One haplotype was shared among the individuals of 6 geographical populations. The F _ (st) value of most populations was higher and the difference was significant, and the degree of genetic differentiation was higher. The results of molecular variance analysis showed that the genetic variation mainly came from the genetic differences within the population. Phylogenetic analysis shows that there are no obvious genetic branches and no strict distribution and differentiation in the studied geographical populations, which may be due to the existence of impermeable hybridization or incomplete pedigree screening among populations. The network diagram also shows that there is no obvious pedigree structure among the sampled populations. The analysis of population history shows that there is no expansion phenomenon in each geographical population. The combination of all geographical populations shows that Bayesian profile analysis and base mismatch analysis both detect rapid population expansion in history, and population expansion occurs before about 0.05 Ma.
【作者單位】： 廣西壯族自治區(qū)藥用植物園;廣西動(dòng)力技工學(xué)校;廣西師范學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31460559) 廣西自然科學(xué)基金項(xiàng)目(2014GXNSFBA118113)
【分類號(hào)】：Q953

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