【摘要】：為了闡明黑龍江省不同地區(qū)的東方次睪吸蟲線粒體細(xì)胞色素C氧化酶第I亞基(cox1)和煙酰胺腺嘌呤二核苷酸脫氫酶亞單位I(nad1)的遺傳變異及種系發(fā)育情況,本研究利用PCR方法擴(kuò)增黑龍江省3個(gè)地區(qū)的東方次睪吸蟲線粒體cox1和nad1部分基因,對(duì)其序列分析并構(gòu)建種系發(fā)生樹,探討其遺傳進(jìn)化關(guān)系。結(jié)果顯示pcox1和pnad1基因片段長(zhǎng)度分別為699 bp和492 bp,且基因長(zhǎng)度均一致。pcox1基因種內(nèi)變異率在0~0.7%,種間變異率在12.2%~15.8%;pnad1基因種內(nèi)變異率在0~0.2%,種間變異率在12.7%~23.3%,種間變異要大于種內(nèi)變異。分子進(jìn)化樹結(jié)果顯示,3個(gè)地區(qū)的東方次睪吸蟲聚集在一起,次睪屬的另兩個(gè)成員聚集在同一分支,且系統(tǒng)發(fā)生樹中的Bootstrap值均較高。本研究表明線粒體cox1基因和nad1基因可以用于東方次睪吸蟲的種系發(fā)育分析,本研究為東方次睪吸蟲進(jìn)一步的分子流行病學(xué)和數(shù)量遺傳學(xué)研究奠定了實(shí)驗(yàn)基礎(chǔ)。
[Abstract]:In order to clarify the genetic variation and species development of mitochondrial cytochrome C oxidase subunit I (cox1) and nicotinamide adenine dinucleotide dehydrogenase subunit I (nad1) in different regions of Heilongjiang Province, the genetic variation of mitochondrial cytochrome C oxidase subunit I and nicotinamide adenine dinucleotide dehydrogenase subunit I (nad1) in different regions of Heilongjiang Province were studied. In this study, partial mitochondrial cox1 and nad1 genes from three regions of Heilongjiang Province were amplified by PCR, and their sequences were analyzed, the phylogenetic tree was constructed, and the genetic and evolutionary relationship was discussed. The results showed that the length of pcox1 and pnad1 genes were 699 bp and 492 bp, respectively. The intraspecific variation rate of pcox1 gene was 0. 7%, and the inter-species variation rate was 12. 2% and 15. 8%, respectively. The intraspecific variation rate of pnad1 gene was 0. 2%, and the inter-species variation rate was 12. 7% / 23. 3%. The inter-species variation was larger than the intraspecific variation. The results of molecular evolutionary tree showed that the two other members of the genus were clustered in the same branch, and the Bootstrap values in phylogenetic tree were higher than those in the phylogenetic tree. This study indicated that mitochondrial cox1 gene and nad1 gene could be used in phylogenetic analysis of Clonorchis orientalis. This study laid an experimental foundation for further molecular epidemiology and quantitative genetics study of Clonorchis orientalis.
【作者單位】： 黑龍江省獸醫(yī)科學(xué)研究所;
【基金】：黑龍江省自然科學(xué)基金(QC2014C033)
【分類號(hào)】：S852.7

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 韓先桂,王佩良;鴨的東方次睪吸蟲病[J];四川畜牧獸醫(yī);2001年02期

2 張正仁;;雛鴨暴發(fā)東方次睪吸蟲病[J];浙江畜牧獸醫(yī);1986年02期

3 陳克強(qiáng);唐文福;姚惠娟;;鴨東方次睪吸蟲病的病理學(xué)觀察[J];上海畜牧獸醫(yī)通訊;1989年06期

4 賁巧云;居維鸞;杭慶華;;群雞暴發(fā)東方次睪吸蟲病[J];上海畜牧獸醫(yī)通訊;1991年02期

5 王裕卿;劉忠誠(chéng);;東方次睪吸蟲在黑龍江省雞體的發(fā)現(xiàn)[J];黑龍江畜牧獸醫(yī);1992年05期

6 申濟(jì)豐;鴨東方次睪吸蟲的診治[J];四川畜禽;1997年05期

7 申濟(jì)豐;鴨東方次睪吸蟲的診治[J];中國(guó)獸醫(yī)寄生蟲病;1998年01期

8 張正仁;;雛鴨發(fā)生東方次睪吸蟲病[J];遼寧畜牧獸醫(yī);1985年06期

9 任農(nóng);雞東方次睪吸蟲病例報(bào)告[J];中國(guó)人獸共患病雜志;1986年04期

10 姚偉民,田慧芳;雞東方次睪吸蟲病的臨床診治[J];中國(guó)獸醫(yī)寄生蟲病;1997年04期

相關(guān)會(huì)議論文 前1條

1 陳浩;高志東;李春榮;;丹頂鶴東方次睪吸蟲病的診斷與治療[A];中國(guó)畜牧獸醫(yī)學(xué)會(huì)家畜寄生蟲學(xué)分會(huì)第九次學(xué)術(shù)研討會(huì)論文摘要集[C];2006年

相關(guān)碩士學(xué)位論文 前2條

1 梁軒;益陽(yáng)市犬弓首蛔蟲感染情況調(diào)查及ITS、cox1基因的多態(tài)性分析[D];湖南農(nóng)業(yè)大學(xué);2015年

2 那璐;東方次睪吸蟲分子種系發(fā)生及PCR檢測(cè)方法的建立[D];黑龍江八一農(nóng)墾大學(xué);2015年

，

本文編號(hào)：2442548