紅麻肌醇加氧酶基因的分離及表達(dá)分析
發(fā)布時(shí)間：2019-03-14 13:05
【摘要】：干旱是限制植物生長分布和影響作物產(chǎn)量形成的重要非生物因素之一,在響應(yīng)干旱脅迫過程中植物細(xì)胞壁發(fā)揮著重要的調(diào)節(jié)作用,肌醇加氧酶是植物細(xì)胞壁合成的前體物質(zhì)UDP-葡萄糖醛酸合成中的關(guān)鍵酶。為研究該基因在紅麻(Hibiscus cannabinus L.)抗旱和耐鹽過程中的作用,通過紅麻轉(zhuǎn)錄組數(shù)據(jù)庫篩選到肌醇加氧酶(myo-inositol oxygenase)基因的核心片段,利用染色體步移技術(shù)獲得肌醇加氧酶基因的c DNA全長,命名為Hc MIOX;生物信息學(xué)分析表明,Hc MIOX基因編碼序列長948 bp,編碼315個(gè)氨基酸,相對(duì)分子量為31.9 ku,等電點(diǎn)為4.75;RT-PCR分析表明,Hc MIOX基因在紅麻根、莖、葉中均有表達(dá),根中表達(dá)量最高,該研究結(jié)果對(duì)分析該基因功能和了解紅麻抗旱的分子機(jī)制具有重要意義。
[Abstract]:Drought is one of the important abiotic factors that limit plant growth distribution and crop yield formation, and plays an important role in the regulation of plant cell wall in response to drought stress. Inositol oxygenase is the key enzyme in the synthesis of UDP- glucuronic acid, the precursor of plant cell wall synthesis. In order to study the gene in kenaf (Hibiscus cannabinus L.) In the process of drought resistance and salt tolerance, the core fragment of inositol oxygenase (myo-inositol oxygenase) gene was screened by kenaf transcript database. The full length of inositol oxygenase gene c DNA, named Hc MIOX;, was obtained by chromosome step transfer technique. Bioinformatics analysis showed that the coding sequence of, Hc MIOX gene was 948 bp, long and encoded 315 amino acids, and the relative molecular weight was 31.9 ku, isoelectric point 4.75; RT-PCR analysis showed that, Hc MIOX gene was expressed in the roots, stems and leaves of kenaf, and the highest expression was found in the roots. The results of this study are of great significance for analyzing the function of the gene and understanding the molecular mechanism of drought resistance of kenaf.
【作者單位】： 上饒師范學(xué)院生命科學(xué)學(xué)院;中國農(nóng)業(yè)科學(xué)院麻類研究所;
【基金】：江西省教育廳科學(xué)技術(shù)研究項(xiàng)目(編號(hào):GJJ151050)) 中國農(nóng)業(yè)科學(xué)院創(chuàng)新工程(編號(hào):ASTIP-IBFC03) 上饒師范學(xué)院2014年博士科研啟動(dòng)基金(編號(hào):001055)
【分類號(hào)】：Q943.2;S563.5
本文編號(hào)：2440007
[Abstract]:Drought is one of the important abiotic factors that limit plant growth distribution and crop yield formation, and plays an important role in the regulation of plant cell wall in response to drought stress. Inositol oxygenase is the key enzyme in the synthesis of UDP- glucuronic acid, the precursor of plant cell wall synthesis. In order to study the gene in kenaf (Hibiscus cannabinus L.) In the process of drought resistance and salt tolerance, the core fragment of inositol oxygenase (myo-inositol oxygenase) gene was screened by kenaf transcript database. The full length of inositol oxygenase gene c DNA, named Hc MIOX;, was obtained by chromosome step transfer technique. Bioinformatics analysis showed that the coding sequence of, Hc MIOX gene was 948 bp, long and encoded 315 amino acids, and the relative molecular weight was 31.9 ku, isoelectric point 4.75; RT-PCR analysis showed that, Hc MIOX gene was expressed in the roots, stems and leaves of kenaf, and the highest expression was found in the roots. The results of this study are of great significance for analyzing the function of the gene and understanding the molecular mechanism of drought resistance of kenaf.
【作者單位】： 上饒師范學(xué)院生命科學(xué)學(xué)院;中國農(nóng)業(yè)科學(xué)院麻類研究所;
【基金】：江西省教育廳科學(xué)技術(shù)研究項(xiàng)目(編號(hào):GJJ151050)) 中國農(nóng)業(yè)科學(xué)院創(chuàng)新工程(編號(hào):ASTIP-IBFC03) 上饒師范學(xué)院2014年博士科研啟動(dòng)基金(編號(hào):001055)
【分類號(hào)】：Q943.2;S563.5
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