【摘要】：為弄清枯草芽孢桿菌HAS對甘蔗黑穗病菌的抑制作用機(jī)理,選取可對多種植物病原真菌有抑制作用的抗菌蛋白TasA基因進(jìn)行克隆與分析。結(jié)果表明:在HAS菌株中含有TasA基因,克隆編碼抗菌蛋白TasA的基因全長,序列分析其有786個堿基組成,該序列與GenBank上登錄的TasA(AJ871386.1)序列同源性達(dá)99%,推測蛋白分子量大小大約28KD,其中第104、164、169、250、399、623、627位等7個堿基發(fā)生堿基轉(zhuǎn)換或顛換,而且這些變異分別發(fā)生在密碼子的第2、2、3、1、3、2、3位堿基上,引起多肽鏈氨基酸的錯義突變。經(jīng)氨基酸序列比對,同Bacillus subtilis subsp.subtilis str.168編碼的TasA蛋白(NP_390342.1)在第150位和第209位上的氨基酸發(fā)生變化,由蘇氨酸、天冬酰胺分別代替丙氨酸和谷氨酸。
[Abstract]:In order to find out the inhibitory mechanism of Bacillus subtilis HAS on the sugarcane smut, the anti-bacterial protein TasA gene, which can inhibit various plant pathogenic fungi, was selected to clone and analyze. The results showed that in the HAS strain, the gene of the TasA gene was cloned and the total length of the gene encoding the antibacterial protein TasA was cloned. The sequence analysis showed that the sequence was up to 99% and the molecular weight of the putative protein was about 28KD. In which 7 bases, such as 104,164,169,250,399,623,627, have base conversion or change, and these variations occur at the 2nd, 2nd, 3rd, 1st, 3rd, 2nd and 3rd bases of the codons, respectively, to cause the missense mutation of the amino acid of the polypeptide chain. The amino acids of the TasA protein (NP-390342.1) encoded by the Bacillus subtilis subsp.subtilis str.168 at the positions 150 and 209 were changed by the amino acid sequence, and the amino acids in the positions 150 and 209 were substituted for alanine and glutamic acid, respectively.
【作者單位】： 中國熱帶農(nóng)業(yè)科學(xué)院熱帶生物技術(shù)研究所/甘蔗研究中心;海南醫(yī)學(xué)院理學(xué)院;
【基金】：國家自然科學(xué)基金(面上項目)“甘蔗黑穗病拮抗菌株HAS抑菌機(jī)理研究”(31471555) 海南省自然科學(xué)基金項目“甘蔗黑穗病拮抗菌株HAS防病機(jī)制研究”(314120) 現(xiàn)代農(nóng)業(yè)產(chǎn)業(yè)技術(shù)體系建設(shè)專項資金甘蔗植保崗位科學(xué)家經(jīng)費(fèi)(nycytx-24)
【分類號】：S476.1;S435.661
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本文編號：2436242