【摘要】：為了揭示蒲公英在干旱脅迫下滲透調(diào)節(jié)和酶保護(hù)系統(tǒng)的作用機(jī)理以及質(zhì)膜水孔蛋白PIP2-3基因的表達(dá)特征,采用盆栽控水試驗(yàn),研究了不同干旱脅迫RWC=(90±5)%、RWC=(75±5)%、RWC=(50±5)%下蒲公英滲透調(diào)節(jié)物質(zhì)、酶保護(hù)系統(tǒng)以及質(zhì)膜水孔蛋白基因的相關(guān)表達(dá)。結(jié)果表明:隨著干旱脅迫的加重,蒲公英葉片中的可溶性糖、可溶性蛋白、游離脯氨酸的含量顯著增加,超氧化物歧化酶(SOD)、過(guò)氧化物酶(POD)、過(guò)氧化氫酶(CAT)的活性顯著提高,在輕度脅迫(MS)中呈持續(xù)上升的趨勢(shì),重度脅迫(SS)中則呈現(xiàn)先升高后下降的趨勢(shì)(可溶性糖除外);質(zhì)膜水孔蛋白PIP2-3基因的相對(duì)表達(dá)量上調(diào),輕度脅迫(MS)顯著高于重度脅迫(SS)。復(fù)水7 d后,滲透調(diào)節(jié)物質(zhì)的積累以及SOD的含量下降,但仍顯著高于CK,POD、CAT的含量以及質(zhì)膜水孔蛋白PIP2-3基因的相對(duì)表達(dá)量恢復(fù)正常,說(shuō)明在干旱脅迫條件下蒲公英通過(guò)增加滲透調(diào)節(jié)物質(zhì)的積累,提高酶保護(hù)系統(tǒng)的活性來(lái)增強(qiáng)植株的抗逆性,上調(diào)質(zhì)膜水孔蛋白基因的相對(duì)表達(dá)量來(lái)加強(qiáng)水分運(yùn)輸能力。
[Abstract]:In order to reveal the mechanism of osmotic regulation and enzyme protection system of dandelion under drought stress and the expression characteristics of plasma membrane aquaporin PIP2-3 gene, the RWC= (90 鹵5)% and RWC= (75 鹵5)% under different drought stress were studied by pot water control experiment. RWC= (50 鹵5)%. The osmotic regulator, enzyme protective system and the expression of aquaporin gene in the plasma membrane of dandelion were studied. The results showed that the contents of soluble sugar, soluble protein and free proline in dandelion leaves increased significantly with the increase of drought stress, and the content of superoxide dismutase (SOD),) peroxidase (POD),) in the leaves of dandelion increased significantly. The activity of catalase (CAT) increased significantly, and the activity of catalase (CAT) increased significantly in mild stress (MS), while in severe stress (SS) increased first and then decreased (except soluble sugar). The relative expression of plasma membrane aquaporin PIP2-3 gene was up-regulated, and the level of (MS) under mild stress was significantly higher than that of (SS). Under severe stress. After 7 days of rehydration, the accumulation of osmotic regulators and the content of SOD decreased, but still significantly higher than the content of CK,POD,CAT and the relative expression of PIP2-3 gene of plasma membrane aquaporin returned to normal. The results showed that dandelion could enhance plant resistance by increasing the accumulation of osmotic regulatory substances, enhancing the activity of enzyme protection system, and increasing the relative expression of plasma membrane aquaporin gene to enhance the water transport ability under drought stress.
【作者單位】： 四川農(nóng)業(yè)大學(xué)園藝學(xué)院;
【基金】：四川省教育廳項(xiàng)目(10ZB044)
【分類(lèi)號(hào)】：S567.239

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