密碼子優(yōu)化BTRCP-CypA融合基因的PCR合成
發(fā)布時(shí)間：2019-02-16 01:21
【摘要】：為了提高BTRCP-Cyp A融合基因的表達(dá)量,對(duì)基因密碼子進(jìn)行優(yōu)化,主要是去除過多富含AT堿基以及提高GC含量。在此基礎(chǔ)上提出一種用于基因合成的PCR法。拆分單鏈寡核首酸長(zhǎng)度為59 nt左右,重疊區(qū)堿基數(shù)14~21 nt,Tm值為46~62℃。具體步驟如下:把拆分的寡核首酸組裝成300~500 bp左右的中間片段,再將中間片段拼接成全長(zhǎng)基因。結(jié)果表明利用該法法合成了BTRCP-Cyp A融合基因,并提高了表達(dá)量。因而改良的PCR法能經(jīng)濟(jì)、簡(jiǎn)便、高效地進(jìn)行基因合成,具有良好的應(yīng)用前景。
[Abstract]:In order to improve the expression of BTRCP-Cyp A fusion gene, the codon of the gene was optimized to remove the excessive amount of AT bases and increase the content of GC. On this basis, a PCR method for gene synthesis is proposed. The length of cleavage oligonucleus was about 59 nt, the base number of overlapping region was 14 ~ 21 nt,Tm and the value was 46 ~ 62 鈩，
本文編號(hào)：2423899
[Abstract]:In order to improve the expression of BTRCP-Cyp A fusion gene, the codon of the gene was optimized to remove the excessive amount of AT bases and increase the content of GC. On this basis, a PCR method for gene synthesis is proposed. The length of cleavage oligonucleus was about 59 nt, the base number of overlapping region was 14 ~ 21 nt,Tm and the value was 46 ~ 62 鈩，
本文編號(hào)：2423899
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