不同葡萄糖轉(zhuǎn)運途徑相關(guān)基因的過表達對菌株葡萄糖代謝及產(chǎn)物合成的影響
發(fā)布時間：2019-01-19 18:35
【摘要】：磷酸烯醇式丙酮酸-葡萄糖磷酸轉(zhuǎn)移酶系統(tǒng)(phosphoenolpyruvate-dependent glucose phosphotransferase system,PTS~(Glc))和不依賴于PTS~(Glc)系統(tǒng)葡萄糖轉(zhuǎn)運系統(tǒng)在葡萄糖轉(zhuǎn)運和磷酸化過程中起重要作用。該文通過在葡萄糖代謝緩慢的L-賴氨酸產(chǎn)生菌Corynebacterium glutamicum(C.glutamicum)ZL-8中過表達肌醇透性酶基因(iol T1)、葡萄糖激酶基因(ppgk)和EIIGlc基因(pts G),研究葡萄糖轉(zhuǎn)運途徑相關(guān)基因?qū)ζ咸烟谴x速率、L-賴氨酸及副產(chǎn)物合成的影響。與出發(fā)菌株C.glutamicum ZL-8相比,重組菌C.glutamicum ZL-8/p ECXK-99E-ppgkiol T1(葡萄糖激酶酶活力提高了768%)和C.glutamicum ZL-8/p DXW-8-pts G(PTS~(Glc)系統(tǒng)酶活力提高了62.7%)葡萄糖消耗速率分別提高了16.7%和27.3%,L-賴氨酸產(chǎn)量分別提高了24.7%和16.4%。此外,有機酸和氨基酸分析結(jié)果表明,過表達不同葡萄糖轉(zhuǎn)運途徑基因?qū)τ袡C酸(丙酮酸、乳酸和乙酸)和副產(chǎn)物氨基酸(丙氨酸、纈氨酸、天冬氨酸)具有不同的影響。
[Abstract]:Phosphoenolpyruvate-glucose phosphotransferase system (phosphoenolpyruvate-dependent glucose phosphotransferase system,PTS~ (Glc) and PTS~ (Glc) independent glucose transport system) plays an important role in the process of glucose transport and phosphorylation. In this paper, inositol permeability enzyme gene (iol T1), glucokinase gene (ppgk) and EIIGlc gene (pts G), were overexpressed in ZL-8 of L- lysine producing bacteria (Corynebacterium glutamicum (C.glutamicum), where glucose metabolism is slow. To study the effects of glucose transporter related genes on glucose metabolism rate, L-lysine and by-product synthesis. Compared with the original strain C.glutamicum ZL-8, Glucose consumption of recombinant bacteria C.glutamicum ZL-8/p ECXK-99E-ppgkiol T1 (increased by 76.8%) and C.glutamicum ZL-8/p DXW-8-pts G (PTS~ (Glc) system increased by 62.7%) The rate increased by 16.7% and 27.3%, respectively. The yield of L-lysine increased by 24. 7% and 16. 4%, respectively. In addition, the analysis of organic acids and amino acids showed that overexpression of different glucose transporter genes had different effects on organic acids (pyruvate, lactic acid and acetic acid) and by-product amino acids (alanine, valine, aspartic acid).
【作者單位】： 江南大學生物工程學院工業(yè)生物技術(shù)教育部重點實驗室;
【基金】：江蘇省自然科學基金青年基金(BK20150149)
【分類號】：TQ922.3
，
本文編號：2411638
[Abstract]:Phosphoenolpyruvate-glucose phosphotransferase system (phosphoenolpyruvate-dependent glucose phosphotransferase system,PTS~ (Glc) and PTS~ (Glc) independent glucose transport system) plays an important role in the process of glucose transport and phosphorylation. In this paper, inositol permeability enzyme gene (iol T1), glucokinase gene (ppgk) and EIIGlc gene (pts G), were overexpressed in ZL-8 of L- lysine producing bacteria (Corynebacterium glutamicum (C.glutamicum), where glucose metabolism is slow. To study the effects of glucose transporter related genes on glucose metabolism rate, L-lysine and by-product synthesis. Compared with the original strain C.glutamicum ZL-8, Glucose consumption of recombinant bacteria C.glutamicum ZL-8/p ECXK-99E-ppgkiol T1 (increased by 76.8%) and C.glutamicum ZL-8/p DXW-8-pts G (PTS~ (Glc) system increased by 62.7%) The rate increased by 16.7% and 27.3%, respectively. The yield of L-lysine increased by 24. 7% and 16. 4%, respectively. In addition, the analysis of organic acids and amino acids showed that overexpression of different glucose transporter genes had different effects on organic acids (pyruvate, lactic acid and acetic acid) and by-product amino acids (alanine, valine, aspartic acid).
【作者單位】： 江南大學生物工程學院工業(yè)生物技術(shù)教育部重點實驗室;
【基金】：江蘇省自然科學基金青年基金(BK20150149)
【分類號】：TQ922.3
，
本文編號：2411638
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