【摘要】：梨品種S基因型鑒定對(duì)梨栽培中授粉品種選擇和遺傳育種都具有重要意義。本研究利用梨S-RNase基因熒光標(biāo)記的特異引物PCR擴(kuò)增獲得梨品種熒光標(biāo)記的cDNA特異產(chǎn)物;進(jìn)一步完善梨S-RNase基因cDNA芯片,以被檢測(cè)梨品種cDNA特異序列與梨S-RNase基因cDNA芯片雜交檢測(cè)不同梨品種S基因型,并發(fā)現(xiàn)新的S-RNase基因。結(jié)果表明:利用梨S-RNase基因cDNA芯片鑒定了瀘定王皮梨、興山24號(hào)、彌渡百合等35個(gè)未知S基因型梨品種,確定了各品種的S基因型。結(jié)合PCRRFLP及DNA克隆和測(cè)序等技術(shù),發(fā)現(xiàn)了7個(gè)新的S-RNase基因資源,獲得了新S-RNase基因序列。序列分析表明各新S-RNase基因均具有S-RNase基因特異區(qū)域序列的典型特征;進(jìn)化分析顯示7個(gè)新S-RNase基因主要屬于薔薇科蘋果亞科S-RNase類群,且存在種間和屬間比種內(nèi)和屬內(nèi)進(jìn)化關(guān)系更近的現(xiàn)象。7個(gè)新的S基因分別命名為:PpS_(53)(Pyrus pyrifolia S53)、PpS_(54)、PpS_(55)、PpS_(56)、PpS_(57)、PpS_(58)和PpS_(59),GenBank登錄號(hào)分別為:KX581753、KX581754、KX581755、KX581756、KX581757、KX581751和KX581752。
[Abstract]:The S genotype identification of pear varieties is of great significance to the selection of pollinated varieties and genetic breeding in pear cultivation. In this study, the fluorescent labeled cDNA specific products of pear varieties were obtained by PCR amplification with fluorescent primers of S-RNase gene. The S-RNase gene cDNA chip was further improved to detect the S-genotypes of different pear cultivars by hybridization of the specific cDNA sequence of the tested pear varieties and the S-RNase gene cDNA chip, and a new S-RNase gene was found. The results showed that 35 unknown S-genotypes, including Luding Wangpi pear, Xingshan 24 and Mudu Lily, were identified by S-RNase gene cDNA chip, and S-genotypes of each variety were determined. In combination with PCRRFLP, DNA cloning and sequencing, seven new S-RNase gene resources were found and new S-RNase gene sequences were obtained. Sequence analysis showed that each new S-RNase gene had typical characteristics of S-RNase gene specific region. Phylogenetic analysis showed that the seven new S-RNase genes mainly belong to the S-RNase group of the Rosaceae subfamily. Seven new S genes were named as PpS_ (53) (Pyrus pyrifolia S53), PpS_ (54), PpS_ (55), PpS_ (56), PpS_ (57). The login numbers for PpS_ (58) and PpS_ (59), GenBank) are KX581753,KX581754,KX581755,KX581756,KX581757,KX581751 and KX581752., respectively
【作者單位】： 湖南工業(yè)大學(xué)包裝與材料工程學(xué)院;中南林業(yè)科技大學(xué)經(jīng)濟(jì)林培育與保護(hù)教育部重點(diǎn)實(shí)驗(yàn)室/經(jīng)濟(jì)林育種與栽培國家林業(yè)局重點(diǎn)實(shí)驗(yàn)室;湖北省農(nóng)業(yè)科學(xué)院果樹茶葉研究所;
【基金】：國家自然科學(xué)基金面上項(xiàng)目(31272124) 國家林業(yè)局林業(yè)科技成果推廣項(xiàng)目([2014]53)
【分類號(hào)】：Q943.2;S661.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前3條

1 周建濤;姜雪婷;王春雷;張紹鈴;;梨花柱S-RNase對(duì)花粉管內(nèi)CaM分布的影響[J];西北植物學(xué)報(bào);2008年06期

2 唐亮;馬香;李明霞;周志欽;;蘋果屬S-RNase基因的進(jìn)化研究[J];中國農(nóng)業(yè)科學(xué);2013年13期

3 ;[J];;年期

，

本文編號(hào)：2406569