穿心蓮甲羥戊酸5-焦磷酸脫羧酶基因的克隆與表達
發(fā)布時間：2019-01-08 18:54
【摘要】：穿心蓮內(nèi)酯是穿心蓮的主要藥效成分,廣泛用于抗炎。為了對它的生物合成進行遺傳調(diào)控,提高內(nèi)酯類成分的合成量,作者在轉(zhuǎn)錄組數(shù)據(jù)分析的基礎(chǔ)上,獲得了穿心蓮內(nèi)酯生物合成途徑中甲羥戊酸5-焦磷酸脫羧酶基因的3個克隆,包含的ORF均長1 260 bp,分別在4個位點具有單堿基的差異。它們編碼由419個殘基組成的氨基酸序列;保守結(jié)構(gòu)域中均具有11個高度保守的氨基酸,分別決定催化反應(yīng)的特異性和活性;N端不含有質(zhì)體定位的信號肽;與丹參的MVD蛋白(Gen Bank號AEZ55675.1)一致性較高。在穿心蓮莖和葉片中,MVD基因的表達量分別在花蕾期和初花期最高,但各時期表達量的倍數(shù)差異不大。該研究獲得的MVD基因,為后續(xù)進行詳細的功能解析,并進一步應(yīng)用于穿心蓮內(nèi)酯的遺傳調(diào)控,奠定了基礎(chǔ)。
[Abstract]:Andrographolide is the main active component of andrographis and widely used in anti-inflammatory. In order to genetically regulate its biosynthesis and improve the synthesis of lactones, the authors based on the analysis of transcriptome data, Three clones of 5 pyrophosphate decarboxylase gene were obtained from andrographis lactone biosynthesis pathway. The ORF contained 1 260 bp, with a single base at each of the four sites. They encode amino acid sequences consisting of 419 residues, 11 highly conserved amino acids in the conserved domain, which determine the specificity and activity of the catalytic reaction respectively. There was high consistency with the MVD protein (Gen Bank (AEZ55675.1) of Salvia miltiorrhiza. In the stems and leaves of andrographis, the expression of MVD gene was the highest in the bud stage and the early flowering stage, but there was no significant difference in the multiple expression of the MVD gene in each stage. The obtained MVD gene provides a basis for further functional analysis and further application in genetic regulation of andrographolide.
【作者單位】： 江西中醫(yī)藥大學江西民族傳統(tǒng)藥現(xiàn)代科技與產(chǎn)業(yè)發(fā)展協(xié)同創(chuàng)新中心;江西省中藥種質(zhì)資源工程技術(shù)研究中心;桂林醫(yī)學院;
【基金】：國家科技支撐計劃項目(2012BAI29B02)
【分類號】：S567.239;Q943.2
本文編號：2404992
[Abstract]:Andrographolide is the main active component of andrographis and widely used in anti-inflammatory. In order to genetically regulate its biosynthesis and improve the synthesis of lactones, the authors based on the analysis of transcriptome data, Three clones of 5 pyrophosphate decarboxylase gene were obtained from andrographis lactone biosynthesis pathway. The ORF contained 1 260 bp, with a single base at each of the four sites. They encode amino acid sequences consisting of 419 residues, 11 highly conserved amino acids in the conserved domain, which determine the specificity and activity of the catalytic reaction respectively. There was high consistency with the MVD protein (Gen Bank (AEZ55675.1) of Salvia miltiorrhiza. In the stems and leaves of andrographis, the expression of MVD gene was the highest in the bud stage and the early flowering stage, but there was no significant difference in the multiple expression of the MVD gene in each stage. The obtained MVD gene provides a basis for further functional analysis and further application in genetic regulation of andrographolide.
【作者單位】： 江西中醫(yī)藥大學江西民族傳統(tǒng)藥現(xiàn)代科技與產(chǎn)業(yè)發(fā)展協(xié)同創(chuàng)新中心;江西省中藥種質(zhì)資源工程技術(shù)研究中心;桂林醫(yī)學院;
【基金】：國家科技支撐計劃項目(2012BAI29B02)
【分類號】：S567.239;Q943.2
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