發(fā)布時(shí)間：2018-12-30 17:47

【摘要】：HKT類基因是與植物耐鹽性密切相關(guān)的一類基因。在作物中HKT蛋白可通過(guò)排出Na+來(lái)維持植物體內(nèi)的Na~+/K~+平衡,從而影響植物耐鹽性。通過(guò)在煙草中過(guò)表達(dá)玉米ZmHKT1;5基因,驗(yàn)證該基因具有提高植物耐鹽性的作用。結(jié)果表明,過(guò)表達(dá)ZmHKT1;5基因的T0代材料即顯示出葉片耐鹽能力的明顯提高;T2代轉(zhuǎn)基因株系種子在含鹽培養(yǎng)基上的發(fā)芽能力明顯強(qiáng)于野生型材料,T2代轉(zhuǎn)基因株系幼苗階段的耐鹽能力也得到了明顯的提高。通過(guò)比較在鹽脅迫后2月齡的轉(zhuǎn)基因材料和野生型材料的生理指標(biāo),發(fā)現(xiàn)野生型材料中MDA和H_2O_2的含量相較轉(zhuǎn)基因材料發(fā)生了更為明顯的上升,說(shuō)明轉(zhuǎn)基因材料中過(guò)表達(dá)ZmHKT1;5基因有效降低了鹽脅迫引起的過(guò)氧化物積累。綜合轉(zhuǎn)基因驗(yàn)證的結(jié)果,證明ZmHKT1;5基因具有提高植物耐鹽性的作用。
[Abstract]:HKT genes are closely related to salt tolerance in plants. HKT protein can maintain the Na~ / K ~ balance in plants by excreting Na, thus affecting the salt tolerance of plants. By overexpressing maize ZmHKT1;5 gene in tobacco, it was proved that this gene could improve plant salt tolerance. The results showed that the T0 generation material which over-expressed ZmHKT1;5 gene showed that the salt-tolerant ability of leaves was obviously improved. The germinating ability of T2 transgenic lines on salt-containing medium was obviously stronger than that of wild-type materials, and the salt tolerance of T2 transgenic lines was also improved obviously in seedling stage. By comparing physiological indexes of transgenic and wild transgenic materials at 2 months of age after salt stress, it was found that the contents of MDA and H_2O_2 in wild materials were significantly higher than those in transgenic materials. It shows that ZmHKT1; is overexpressed in transgenic materials. 5 gene effectively reduced peroxide accumulation induced by salt stress. The results showed that ZmHKT1;5 gene could improve the salt tolerance of plants.
【作者單位】： 吉林省農(nóng)業(yè)科學(xué)院農(nóng)業(yè)生物技術(shù)研究所/吉林省農(nóng)業(yè)生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(31100242)
【分類號(hào)】：Q943.2;S513

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