【摘要】：旨在探究不同營養(yǎng)水平及不同生長階段脂尾型綿羊NELL1和FMO3基因mRNA的組織特異性表達(dá)模式。選擇健康、體質(zhì)量相近的9月齡灘羊、同羊、哈薩克羊和西藏羊(羯羊)各3只,活體采集尾部脂肪樣品。選擇健康4月齡灘羊112只隨機(jī)分為4組,公母各半,每組4個重復(fù),每個重復(fù)7只羊。根據(jù)體質(zhì)量增加目標(biāo)分為3個階段:22~28kg,28~35kg,35~40kg,每個階段各組分別飼喂不同營養(yǎng)水平的日糧。每個生長階段結(jié)束時采集肝臟、腎周脂肪、尾脂、皮下脂肪的組織樣品,提取樣品總RNA,采用實(shí)時熒光定量PCR的方法分析NELL1和FMO3 mRNA的表達(dá)差異。結(jié)果表明,FMO3 mRNA在哈薩克羊尾脂中的表達(dá)量最高,在同羊、灘羊、西藏羊尾脂中依次下降,且差異顯著;NELL1 mRNA在西藏羊尾脂中的表達(dá)量最高,在灘羊、同羊、哈薩克羊中的表達(dá)量依次下降;營養(yǎng)水平和月齡對尾脂和肝臟中NELL1和FMO3 mRNA的表達(dá)水平有顯著影響,而對皮下脂肪和腎周脂肪中NELL1和FMO3 mRNA的表達(dá)影響不顯著。表明FMO3mRNA的表達(dá)水平與脂肪沉積呈正相關(guān),而NELL1 mRNA的表達(dá)水平與脂肪沉積呈負(fù)相關(guān);NELL1與FMO3對綿羊尾脂和肝臟中的脂肪沉積起調(diào)控作用,為進(jìn)一步揭示脂尾型綿羊體內(nèi)脂肪沉積的調(diào)控機(jī)制提供理論基礎(chǔ)。
[Abstract]:The aim of this study was to investigate the tissue specific expression patterns of NELL1 and FMO3 gene mRNA in fat-tailed sheep at different nutritional levels and different growth stages. Tail-fat samples were collected from 9 month old Tan sheep, 3 Kazak sheep and 3 Tibetan sheep (Capricorn), which were healthy and of similar body weight. 112 healthy 4 months old Tan sheep were randomly divided into 4 groups, male and female were divided into 4 groups, each group had 4 replicates, each group had 7 sheep. According to the objective of increasing body mass, there were three stages: 22 ~ 28kg ~ 28kg ~ (-1) ~ 2835kg ~ (35) kg ~ (-1) ~ (35) kg ~ (35) ~ (40) kg, and each group was fed with different nutrition level. Tissue samples of liver, perirenal fat, tail fat and subcutaneous fat were collected at the end of each growth stage. The expression differences between NELL1 and FMO3 mRNA were analyzed by real-time fluorescence quantitative PCR. The results showed that the expression of FMO3 mRNA was the highest in Kazak sheep tail fat, and decreased in Tongyang sheep, Tan sheep and Tibetan sheep tail fat, and the difference was significant. The expression of NELL1 mRNA was the highest in Tibetan sheep tail fat, and decreased in Tan sheep, Tong sheep and Kazak sheep. Nutritional level and age had significant effects on the expression of NELL1 and FMO3 mRNA in tail fat and liver, but had no significant effect on the expression of NELL1 and FMO3 mRNA in subcutaneous fat and perirenal fat. The results showed that the expression of FMO3mRNA was positively correlated with fat deposition, while the expression of NELL1 mRNA was negatively correlated with fat deposition. NELL1 and FMO3 play an important role in regulating fat deposition in sheep tail fat and liver, which provides a theoretical basis for further revealing the regulation mechanism of lipid tail fat deposition in sheep.
【作者單位】： 西北農(nóng)林科技大學(xué)動物科技學(xué)院;
【基金】：國家絨毛用羊產(chǎn)業(yè)技術(shù)體系(CARS-40-13) 公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201303059) 陜西省科學(xué)技術(shù)研究發(fā)展計(jì)劃(2014K01-17-03) 陜西省農(nóng)業(yè)科技攻關(guān)(2014K01-17-04)~~
【分類號】：S826

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