【摘要】：MAPKKK(mitogen-activated protein kinase kinase kinase)在植物抗逆脅迫中起重要作用。為分析花生中MAPKKK基因的情況,本試驗(yàn)對耐鹽花生(Arachis hypogaea)突變體(S2)和對照(S4)的葉片進(jìn)行RNA測序分析,篩選出了19個具有完整開放閱讀框的MAPKKK基因。它們位于花生A組野生種的8條染色體上,不同成員的氨基酸殘基個數(shù)、外顯子數(shù)目差異很大。聚類分析表明19個花生MAPKKK基因能聚到MEKK和Raf兩個亞家族。我們利用S2和S4構(gòu)建了鹽脅迫處理前后的表達(dá)譜,篩選出4個受鹽脅迫誘導(dǎo)表達(dá)的MAPKKK基因,分別屬于MEKK亞家族(3個)和Raf亞家族(1個)。該研究為花生MAPKKK基因的功能研究和利用提供參考。
[Abstract]:MAPKKK (mitogen-activated protein kinase) plays an important role in plant stress resistance. In order to analyze the situation of MAPKKK gene in peanut, 19 MAPKKK genes with complete open reading frame were screened by RNA sequencing in leaves of salt-tolerant peanut (Arachis hypogaea) mutant (S2) and control (S4). They are located on 8 chromosomes of group A wild species of peanut. The number of amino acid residues and exons of different members vary greatly. Cluster analysis showed that 19 peanut MAPKKK genes could be clustered into two subfamilies of MEKK and Raf. We constructed the expression profiles before and after salt stress with S2 and S4, and screened out four MAPKKK genes induced by salt stress, belonging to MEKK subfamily (3) and Raf subfamily (1) respectively. This study provides a reference for the functional study and utilization of peanut MAPKKK gene.
【作者單位】： 青島農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院山東省高校植物生物技術(shù)重點(diǎn)實(shí)驗(yàn)室;山東省農(nóng)業(yè)技術(shù)推廣總站;泰山學(xué)院生物與釀酒工程學(xué)院;
【基金】：國家自然科學(xué)基金(31571705;31301356和31471542) 山東省科技發(fā)展計(jì)劃(2014GNC110002) 泰安市科技局項(xiàng)目(20132088)~~
【分類號】：S565.2

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1 寧婧;水稻中兩個MAPKKK基因的功能鑒定[D];華中農(nóng)業(yè)大學(xué);2010年

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本文編號：2386617