【摘要】：溫度和鹽度分別作為海洋環(huán)境中基礎(chǔ)又重要的環(huán)境因素之一,對(duì)海洋浮游微藻的生長(zhǎng)有重要的影響。促有絲分裂活化蛋白激酶(mitogen-activated protein kinase,MAPK)是信號(hào)傳導(dǎo)通路的關(guān)鍵分子,存在于真核生物中,它對(duì)于植物的生長(zhǎng)發(fā)育調(diào)節(jié)及抗逆應(yīng)答等生理過(guò)程起到了重要的作用。為了深入探討有害藻華生物對(duì)溫度和鹽度變化的分子響應(yīng),本文以MAPK基因作為分析對(duì)象,以我國(guó)近海常見(jiàn)的有害藻華原因種米氏凱倫藻(Karenia mikimotoi)為研究目標(biāo)。選擇不同來(lái)源的兩株米氏凱倫藻藻株:惠州株和福建株,分別培養(yǎng)在溫度和鹽度逐漸變化的環(huán)境中(溫度的變化范圍為15-33℃,鹽度變化范圍為15-38),從藻細(xì)胞的比生長(zhǎng)速率、葉綠素a濃度、最大潛在光合能力及分子生物學(xué)等方面,探究?jī)芍昝资蟿P倫藻對(duì)于溫度和鹽度變化的適應(yīng)性,以及溫度和鹽度變化對(duì)MAPK基因表達(dá)的影響。本研究取得的主要研究結(jié)果如下:1.米氏凱倫藻福建株的溫度適應(yīng)范圍是15-30℃,鹽度適應(yīng)范圍是20-37,最適溫度范圍是24-28℃,最適鹽度范圍是23-37。米氏凱倫藻惠州株的溫度適應(yīng)范圍是15-33℃,鹽度適應(yīng)范圍是20-37,最適溫度范圍是24-30℃,最適鹽度范圍是26-32。2.在溫度變化實(shí)驗(yàn)中,米氏凱倫藻福建株在28℃時(shí)達(dá)到最大比生長(zhǎng)速率,為0.37d~(-1);而惠州株在26℃時(shí)達(dá)到最大比生長(zhǎng)速率,為0.31d~(-1)。在鹽度變化實(shí)驗(yàn)中,米氏凱倫藻福建株在23時(shí)達(dá)到最大比生長(zhǎng)速率,為0.34d~(-1),而米氏凱倫藻惠州株在26時(shí)達(dá)到最大比生長(zhǎng)速率,為0.20d~(-1)。3.葉綠素a濃度和最大潛在光合能力Fv/Fm值均在一定程度上反映了兩株米氏凱倫藻的生長(zhǎng)狀態(tài)。其中葉綠素a濃度與藻細(xì)胞密度成正相關(guān)關(guān)系。米氏凱倫藻福建株的Fv/Fm值在溫度≥30℃時(shí)受到抑制,在鹽度≥37和≤20時(shí)受到抑制。米氏凱倫藻惠州株的Fv/Fm值在溫度≥28℃和≤20℃時(shí)受到抑制,在鹽度在≥38和≤17時(shí)受到抑制。4.米氏凱倫藻惠州株在22和26℃時(shí)MAPK基因顯著上調(diào),在20和28℃時(shí)MAPK基因顯著下調(diào)。福建株在15和33℃時(shí)MAPK基因顯著下調(diào),在20、26、28和30℃時(shí)MAPK基因顯著上調(diào)。米氏凱倫藻惠州株在鹽度30~37時(shí)MAPK基因顯著下調(diào),在鹽度17和20時(shí)MAPK基因相對(duì)表達(dá)量顯著上調(diào)。福建株在鹽度23、26、30和32時(shí)MAPK基因顯著上調(diào),在34和37時(shí)MAPK基因顯著下調(diào)。結(jié)果表明MAPK基因與米氏凱倫藻適應(yīng)溫度和鹽度變化的調(diào)控相關(guān),說(shuō)明MAPK基因參與了對(duì)外界溫度和鹽度變化信號(hào)的轉(zhuǎn)導(dǎo)過(guò)程。
[Abstract]:As one of the basic and important environmental factors in marine environment, temperature and salinity play an important role in the growth of marine planktonic microalgae. Mitogenic activated protein kinase (mitogen-activated protein kinase,MAPK) is a key signal transduction pathway in eukaryotes and plays an important role in plant growth and development regulation and stress resistance response. In order to study the molecular response of harmful algal blooms to temperature and salinity changes, MAPK gene was used as an analysis object, and (Karenia mikimotoi), a common species of harmful algal blooms, was used as the research object in this paper. Two strains of Karen Michaelis from different sources, Huizhou strain and Fujian strain, were cultured in a gradually changing environment of temperature and salinity (temperature range 15-33 鈩，

本文編號(hào)：2385252