【摘要】：目的分析絕經(jīng)前后低骨密度、高骨密度女性外周血單核細胞基因表達譜,探討外周血單核細胞差異表達基因?qū)Φ凸敲芏�、高骨密度人群的鑒別診斷價值。方法下載公眾數(shù)據(jù)平臺美國國家生物技術(shù)信息中心(National Center of Biotechnology Information,NCBI)下的基因芯片原始數(shù)據(jù)集NCBI-GEO Data Sets-GSE56815,運用R/Bionconductor分析原始數(shù)據(jù),選取差異倍數(shù)1.5,P0.05的基因為差異表達基因;運用Medcalc及SPSS統(tǒng)計軟件對差異表達基因繪制受試者工作特征曲線,分析單一基因檢測及聯(lián)合基因檢測對低骨密度、高骨密度人群的鑒別診斷效果。結(jié)果在絕經(jīng)前女性中,低骨密度、高骨密度組外周血單核細胞存在著58個差異基因(18基因上調(diào)、40基因下調(diào)),且FOLR3ITGB3ABOIGHM聯(lián)合檢測鑒別診斷不同骨密度女性有較好的特異性、敏感性;在絕經(jīng)后女性中,低骨密度、高骨密度組外周血單核細胞存在著75個差異基因(21基因上調(diào),54基因下調(diào)),且TACSTD2MMP8LILRA5聯(lián)合檢測鑒別診斷不同骨密度女性有較好的特異性、敏感性。結(jié)論絕經(jīng)前后女性外周血單核細胞基因表達譜不同,低骨密度組與高骨密度組外周血單核細胞基因表達譜在絕經(jīng)后女性中差異變化更為顯著,外周血單核細胞差異基因聯(lián)合檢測能夠提高對不同骨密度女性的鑒別診斷能力。
[Abstract]:Objective to analyze the gene expression profiles of peripheral blood monocytes in premenopausal and premenopausal women with low BMD and high BMD, and to explore the differential diagnosis of PBMC differentially expressed genes in patients with low BMD and high BMD. Methods the original data set of gene chip was downloaded from (National Center of Biotechnology Information,NCBI (National Biotechnology Information Center), a public data platform. R/Bionconductor was used to analyze the original data, and the multiple of difference was 1.5. P05 genes were differentially expressed genes. Medcalc and SPSS software were used to draw the operating characteristic curves of the differentially expressed genes, and to analyze the effect of single gene detection and combined gene detection on the differential diagnosis of low BMD and high BMD population. Results among premenopausal women, 58 differentially expressed genes (18 genes up-regulated, 40 genes down-regulated) were found in peripheral blood mononuclear cells with low BMD and high BMD, and the differential diagnosis of BMD in women with different BMD by FOLR3ITGB3ABOIGHM combined with BMD was more specific. Sensitivity; In postmenopausal women, 75 differentially expressed genes were found in peripheral blood monocytes of low BMD and high BMD groups (21 gene up-regulated, 54 down-regulated), and TACSTD2MMP8LILRA5 combined detection for differential diagnosis of different BMD women had better specificity and sensitivity. Conclusion the gene expression profiles of peripheral blood monocytes in premenopausal and postmenopausal women are different, and the gene expression profiles of peripheral blood monocytes in low BMD group and high BMD group are more significant in postmenopausal women. The differential gene detection of peripheral blood monocytes can improve the differential diagnosis ability of women with different bone mineral density.
【作者單位】： 西安交通大學(xué)醫(yī)學(xué)院附屬紅會醫(yī)院檢驗科;電信科學(xué)技術(shù)第十研究所;
【基金】：西安市紅會醫(yī)院科研基金資助課題(YJ2016013)
【分類號】：R580;R440

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