【摘要】：為了探討雞粒細(xì)胞-巨噬細(xì)胞集落刺激因子(GM-CSF)基因?qū)﹄u傳染性法氏囊病病毒(IBDV)VP2 DNA疫苗誘導(dǎo)的免疫反應(yīng)的影響,將pTriEx-4-VP2-GM-CSF質(zhì)粒免疫SPF雞,加強(qiáng)免疫2周后,進(jìn)行IBDV強(qiáng)毒攻擊,檢測(cè)機(jī)體免疫相關(guān)指標(biāo)。結(jié)果表明:雞GM-CSF基因能夠增強(qiáng)VP2 DNA疫苗誘導(dǎo)的特異性免疫反應(yīng),影響細(xì)胞因子TNF-α、IFN-γ、IL-2、IL-4、IL-5、IL-6、IL-10的表達(dá)水平,對(duì)外周血淋巴細(xì)胞的增殖沒有顯著影響。這一結(jié)果提示雞GM-CSF基因?qū)BDV VP2 DNA疫苗具有一定的免疫調(diào)節(jié)作用。
[Abstract]:To investigate the effect of granulocyte-macrophage colony stimulating factor (GM-CSF) gene on immune response induced by (IBDV) VP2 DNA vaccine of infectious bursal disease virus (IBDV), pTriEx-4-VP2-GM-CSF plasmid was immunized with SPF chicken. After 2 weeks of intensified immunization, the IBDV virulent attack was carried out, and the immune-related indexes were detected. The results showed that the chicken GM-CSF gene could enhance the specific immune response induced by VP2 DNA vaccine and affect the expression of cytokines TNF- 偽, IFN- 緯 and IL-2,IL-4,IL-5,IL-6,IL-10. There was no significant effect on the proliferation of peripheral blood lymphocytes. These results suggest that chicken GM-CSF gene has some immunomodulatory effect on IBDV VP2 DNA vaccine.
【作者單位】： 廣東溫氏大華農(nóng)生物科技有限公司;華南農(nóng)業(yè)大學(xué)獸醫(yī)學(xué)院;
【基金】：廣東省應(yīng)用型科技研發(fā)專項(xiàng)資金項(xiàng)目(2015B020230011)
【分類號(hào)】：S858.31

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