4個線粒體基因在鯉所屬國家種質(zhì)鑒定中的應(yīng)用及效果評價
發(fā)布時間：2018-11-28 07:48
【摘要】：近年來,mtDNA條形碼技術(shù)已廣泛應(yīng)用于魚類種質(zhì)鑒定研究。為探討該技術(shù)在鯉種質(zhì)鑒定方面應(yīng)用的可行性及如何有效應(yīng)用于地域種群等鑒定分析,從NCBI網(wǎng)站(http://www.ncbi.nlm.nih.gov/)搜集鯉COⅠ、COⅡ、Cytb和D-loop4個mtDNA序列,并嘗試基于DNA條形碼技術(shù)來進(jìn)行鯉所屬國家的鑒定分析。結(jié)果表明:Cytb相對其他3個線粒體序列存在較大的DNA條形碼間隙,COⅡ基因最佳匹配的準(zhǔn)確率最高(準(zhǔn)確率82.97%),基于4個mtDNA序列的聚類分析表明鯉所屬國家按照臨近區(qū)域聚類。綜合條形碼間隙及假陽性率和假陰性率的評價,研究認(rèn)為,相比其他3個mtDNA序列,Cytb基因用于鑒定鯉所屬國家更具優(yōu)勢,但其鑒定效率仍不理想。綜上所述,mtDNA序列用于鑒定鯉不同地域的技術(shù)依然需要改進(jìn),以降低這一技術(shù)的假陽性率和假陰性率。
[Abstract]:In recent years, mtDNA bar code technology has been widely used in fish germplasm identification. In order to explore the feasibility of applying this technique to the identification of common carp germplasm and how to effectively apply it to the identification and analysis of regional populations, the mtDNA sequences of common carp CO 鈪，
本文編號：2362261
[Abstract]:In recent years, mtDNA bar code technology has been widely used in fish germplasm identification. In order to explore the feasibility of applying this technique to the identification of common carp germplasm and how to effectively apply it to the identification and analysis of regional populations, the mtDNA sequences of common carp CO 鈪，
本文編號：2362261
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