發(fā)布時(shí)間：2018-11-20 16:02

【摘要】：釩是一種重金屬,過量的攝入會(huì)造成蛋雞氧化應(yīng)激,降低雞蛋品質(zhì)并殘留到雞蛋中,影響雞蛋的安全。本研究主要通過給大鼠飼喂含釩的蛋黃粉,考察其對(duì)大鼠生長(zhǎng)性能、氧化應(yīng)激狀態(tài)及其相關(guān)基因表達(dá)的影響,為評(píng)價(jià)釩(有機(jī)釩)的生物安全性提供依據(jù)。選用27只4周齡雌性Wistar大鼠單籠飼養(yǎng),設(shè)3個(gè)處理,每個(gè)處理9只,分別在飼糧中添加600 g/kg采食3種釩水平(0、5和10 mg/kg)飼糧的蛋雞所產(chǎn)雞蛋制備的蛋黃粉。經(jīng)實(shí)測(cè),3種飼糧分別含釩0.107、0.137和0.164 mg/kg。試驗(yàn)期35 d。結(jié)果表明:3個(gè)處理大鼠生長(zhǎng)性能、器官指數(shù),血漿甘油三酯、丙二醛(MDA)、尿素氮含量及谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)活性,肝臟、腎臟超氧化物歧化酶活性、MDA含量、總抗氧化能力,肝臟ALT、AST活性、谷胱甘肽含量和谷胱甘肽巰基轉(zhuǎn)移酶活性,以及肝臟、腎臟組織結(jié)構(gòu)、腎臟釩殘留量均無顯著差異(P0.05)。與處理1相比,處理2、3大鼠肝臟醌氧化還原酶1(NQO1)活性顯著下降(P0.05),NQO1和核因子E2相關(guān)因子2 mRNA相對(duì)表達(dá)量也顯著下降(P0.05)。結(jié)果提示:大鼠飼糧中添加600 mg/kg不同釩含量的蛋黃粉對(duì)大鼠生長(zhǎng)性能和機(jī)體氧化還原狀態(tài)的影響無顯著差異,但含釩0.137、0.164 mg/kg可以降低NQO1酶活性并下調(diào)抗氧化應(yīng)答相關(guān)基因的表達(dá)。
[Abstract]:Vanadium is a heavy metal. Excessive intake of vanadium can lead to oxidative stress, reduce the quality of eggs and leave them in eggs, which will affect the safety of eggs. In order to evaluate the biological safety of vanadium (organic vanadium), the effects of vanadium-containing egg yolk powder on growth performance, oxidative stress state and related gene expression in rats were investigated in this study. Twenty-seven 4-week-old female Wistar rats were raised in a single cage and treated with 3 treatments (9 rats each). The egg yolk powder was fed on three kinds of vanadium (0 5 and 10 mg/kg) diets for 600 g/kg, respectively. The measured results show that the three diets contain 0.107 mg/kg. of vanadium and 0.164 mg/kg. of vanadium, respectively. The trial period was 35 days. The results showed that the growth performance, organ index, plasma triglyceride, malondialdehyde (MDA) (MDA), urea nitrogen content, glutamate-alanine aminotransferase (ALT),) -aminotransferase (AST) activity, liver and kidney superoxide dismutase (SOD) activity were measured in three groups. There was no significant difference in MDA content, total antioxidant capacity, liver ALT,AST activity, glutathione content and glutathione mercaptotransferase activity, as well as liver, kidney tissue structure and kidney vanadium residue (P0.05). Compared with treatment 1, the activity of quinone redox enzyme 1 (NQO1) and the relative expression of NQO1 and E2 related factor 2 mRNA were significantly decreased (P0.05). The results showed that there was no significant difference in growth performance and redox state of rats after adding 600 mg/kg different vanadium content of egg yolk powder in diet. However, vanadium 0.137 and 0.164 mg/kg could decrease the activity of NQO1 and down-regulate the expression of genes related to antioxidant response.
【作者單位】： 四川農(nóng)業(yè)大學(xué)動(dòng)物營(yíng)養(yǎng)所教育部抗病營(yíng)養(yǎng)重點(diǎn)實(shí)驗(yàn)室;
【基金】：四川省科技支撐項(xiàng)目(2011NZ0073,2014NZ0002) 科技部科技支撐項(xiàng)目(2014BAD13B04) 四川省教育廳項(xiàng)目(13ZB0290)
【分類號(hào)】：S816

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