擬南芥開花抑制基因TFL1的蛋白表達及純化
發(fā)布時間：2018-11-18 16:42
【摘要】：TFL1基因是植物中的一個開花抑制基因,與促進植物開花的FT是同源基因,但是二者發(fā)揮相反的功能。本研究為了獲得TFL1融合蛋白,首先將TFL1基因構(gòu)建到PGEX-4T-3表達載體上,獲得TFL1基因的原核表達載體PGEX-4T-3-GST-TFL1,轉(zhuǎn)化大腸桿菌表達菌株BL-21(DE3)。在37℃,0.5mmol/L IPTG條件下誘導(dǎo)出了大小為46kD的GST-TFL1融合蛋白。經(jīng)過GST純化基質(zhì)純化后,獲得了質(zhì)量較高、并且能被TFL1抗體特異識別的GST-TFL1蛋白,為進一步研究TFL1的互作蛋白及其抑制植物開花的分子機制提供了一個十分有力的工具。
[Abstract]:TFL1 gene is a flowering suppressor gene in plants. It is a homologous gene with FT which promotes flowering in plants, but they play an opposite function. In order to obtain the TFL1 fusion protein, the TFL1 gene was first constructed into the PGEX-4T-3 expression vector, and the prokaryotic expression vector PGEX-4T-3-GST-TFL1, of TFL1 gene was obtained to transform the E. coli expression strain BL-21 (DE3). The GST-TFL1 fusion protein of 46kD was induced under the condition of 0.5mmol/L IPTG at 37 鈩，
本文編號：2340558
[Abstract]:TFL1 gene is a flowering suppressor gene in plants. It is a homologous gene with FT which promotes flowering in plants, but they play an opposite function. In order to obtain the TFL1 fusion protein, the TFL1 gene was first constructed into the PGEX-4T-3 expression vector, and the prokaryotic expression vector PGEX-4T-3-GST-TFL1, of TFL1 gene was obtained to transform the E. coli expression strain BL-21 (DE3). The GST-TFL1 fusion protein of 46kD was induced under the condition of 0.5mmol/L IPTG at 37 鈩，
本文編號：2340558
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