【摘要】：本研究以不同高度的慈竹筍和當年生慈竹為材料,采用酶聯(lián)免疫法和轉(zhuǎn)錄組測序技術,對慈竹筍生長過程中內(nèi)源激素含量動態(tài)積累和脫落酸(ABA)、赤霉素(GA)與生長素(IAA)KEGG代謝途徑相關基因差異表達進行分析。結(jié)果表明,慈竹在竹筍整個生長過程中,ABA、GA和IAA總含量隨著株高的增高呈現(xiàn)不同的變化趨勢,GA總含量變化呈"S"型曲線,IAA總含量變化呈雙峰曲線,而ABA含量變化不明顯;KEGG代謝途徑中相關基因存在差異表達,其中ABA代謝途徑中PSY和BCH在筍50 cm時呈現(xiàn)上調(diào)表達,ZE在150 cm時呈現(xiàn)下調(diào)表達。GA代謝途徑中KAO與CPS在早期呈現(xiàn)上調(diào)表達,后呈現(xiàn)下調(diào)表達。IAA代謝途徑中CATB在筍生長到50 cm呈現(xiàn)上調(diào)表達,而后呈現(xiàn)下調(diào)表達的趨勢,而ALDH和OGDC隨著筍的伸長呈現(xiàn)表達上調(diào)。
[Abstract]:In this study, the dynamic accumulation of endogenous hormones and abscisic acid (ABA),) in the growth process of amaranthin bamboo shoots were studied by using enzyme linked immunosorbent assay (Elisa) and transcriptional sequencing techniques. The differentially expressed genes related to the metabolic pathway of gibberellin (GA) and auxin (IAA) KEGG were analyzed. The results showed that the total contents of ABA,GA and IAA varied with the increase of plant height during the whole growth process of Phyllostachys sinensis. The total content of GA showed "S" curve, and the total content of IAA showed a double peak curve. However, the content of ABA did not change obviously. The expression of PSY and BCH in the metabolic pathway of ABA was up-regulated at 50 cm, the expression of ZE was down-regulated at 150 cm, and the expression of KAO and CPS was up-regulated in the early stage of GA pathway. In the metabolic pathway of IAA, the expression of CATB was up-regulated at 50 cm and then down-regulated, while the expression of ALDH and OGDC increased with the elongation of shoot.
【作者單位】： 西南科技大學植物細胞工程實驗室;四川省生物質(zhì)資源利用與改性工程技術研究中心;
【基金】：大學生創(chuàng)新創(chuàng)業(yè)項目(201510619020)資助
【分類號】：Q943.2;S795.5

【相似文獻】

相關期刊論文 前1條

1 楊樺;楊茂發(fā);楊偉;楊春平;朱天輝;黃瓊;趙曉英;;竹橫錐大象對寄主及蟲體揮發(fā)物的行為和觸角電位反應[J];昆蟲學報;2010年03期

相關重要報紙文章 前1條

1 小柯;青筍 百般滋味皆于此[N];北京科技報;2003年

，

本文編號：2338988