發(fā)布時(shí)間：2018-11-15 11:14

【摘要】：組氨醇脫氫酶將組氨醇氧化成組氨酸,是組氨酸合成途徑中的最后一步,調(diào)控植物的生長(zhǎng)和發(fā)育過程。為了更好地理解大葉落地生根中胎生苗調(diào)控的分子機(jī)制,利用RACE-PCR技術(shù)從大葉落地生根中克隆了1個(gè)新的組氨醇脫氫酶基因KdHDH。該基因編碼的蛋白質(zhì)具有483個(gè)氨基酸殘基,分子量為52 130,等電點(diǎn)為5.78,其開放閱讀框長(zhǎng)度為1 452 bp。KdHDH蛋白與葡萄、擬南芥、蓖麻等植物的蛋白相似性都很高,聚類分析結(jié)果表明該蛋白與甜菜和菠菜的遺傳距離最近。實(shí)時(shí)熒光定量PCR分析結(jié)果表明,該蛋白基因在大葉落地生根的根中表達(dá)量最高,且受滲透脅迫(甘露醇)的誘導(dǎo)下調(diào)表達(dá)。
[Abstract]:Histidine dehydrogenase oxidizes histidine to histidine which is the last step in histidine synthesis and regulates the growth and development of plants. In order to better understand the molecular mechanism of embryogenic seedling regulation in root formation, a new histamine dehydrogenase gene (KdHDH.) was cloned from the rooting of large leaf landroot by RACE-PCR technique. The protein encoded by this gene has a molecular weight of 52,130 and a isoelectric point of 5.78. The open reading frame length of 1 452 bp.KdHDH protein is similar to that of grape, Arabidopsis thaliana, castor and other plants. The results of cluster analysis showed that the genetic distance between the protein and beet and spinach was the closest. The results of real-time fluorescence quantitative PCR analysis showed that the expression of the protein gene was the highest in the rooting roots, and the expression was down-regulated by osmotic stress (mannitol).
【作者單位】： 深圳市日f園林綠化有限公司;暨南大學(xué)深圳旅游學(xué)院;北京林業(yè)大學(xué)林學(xué)院草坪研究所;華南農(nóng)業(yè)大學(xué)林學(xué)與風(fēng)景園林學(xué)院草業(yè)科學(xué)系/廣東省草業(yè)工程技術(shù)研究中心;
【基金】：深圳市知識(shí)創(chuàng)新項(xiàng)目(基20160093) 北京林業(yè)大學(xué)與廈門日懋城建園林建設(shè)股份有限公司產(chǎn)學(xué)研合作項(xiàng)目
【分類號(hào)】：Q943.2

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