【摘要】：冰緣植物高山離子芥具有極強(qiáng)的抗寒能力,具葶離子芥為其同屬近緣種,但抗寒性遠(yuǎn)不及高山離子芥。本試驗(yàn)以高山離子芥和具葶離子芥植株為材料,對(duì)這2種離子芥進(jìn)行低溫處理,并采用c DNA擴(kuò)增片段長(zhǎng)度多態(tài)性(c DNA-AFLP)技術(shù)對(duì)其進(jìn)行耐冷相關(guān)基因篩選,共獲得與耐冷相關(guān)的28條差異表達(dá)片段,其中7條來(lái)自具葶離子芥,21條來(lái)自高山離子芥,并對(duì)這28條差異表達(dá)的片段進(jìn)行生物信息學(xué)分析。選取高山離子芥特有的5條差異表達(dá)片段(TDFs),采用Realtime RT-PCR進(jìn)行低溫誘導(dǎo)表達(dá)驗(yàn)證,結(jié)果表明確實(shí)是低溫誘導(dǎo)的特異表達(dá)片段。本試驗(yàn)結(jié)果為高山離子芥特有耐冷基因的功能研究及其應(yīng)用提供了依據(jù)。
[Abstract]:Alpine ion mustard has strong cold resistance, and scape ion mustard is a relative species, but its cold resistance is not as good as that of alpine ion mustard. In this experiment, the plants of Alpine ion mustard and scape ion mustard were treated at low temperature, and their cold tolerance genes were screened by c DNA amplified fragment length polymorphism (c DNA-AFLP) technique. A total of 28 differentially expressed fragments related to cold tolerance were obtained, of which 7 were from scape ion mustard and 21 from alpine ion mustard. The 28 differentially expressed fragments were analyzed by bioinformatics. Five differentially expressed fragments (TDFs), were selected for low-temperature induced expression verification by Realtime RT-PCR. The results showed that they were indeed specific expression fragments induced by hypothermia. The results of this study provide a basis for the functional study and application of specific cold tolerance genes in Alpine ion mustard.
【作者單位】： 新疆大學(xué)生命科學(xué)與技術(shù)學(xué)院;伊犁師范學(xué)院生物與地理科學(xué)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金項(xiàng)目(31160051、C020407) 新疆維吾爾自治區(qū)重大科技專(zhuān)項(xiàng)項(xiàng)目(200840102-40)
【分類(lèi)號(hào)】：Q943.2

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