【摘要】：目的采用全基因組表達(dá)譜芯片數(shù)據(jù)分析五子衍宗丸提高大鼠睪丸支持細(xì)胞抵抗熱應(yīng)激能力的作用機(jī)制。方法將原代培養(yǎng)的睪丸支持細(xì)胞分為五子衍宗丸組、正常組和模型組,分別給予五子衍宗丸溶液和等體積的PBS,培養(yǎng)24 h后,五子衍宗丸組和模型組給予43℃水浴15 min,正常組給予37℃水浴15 min處理。分別提取3組細(xì)胞總RNA,通過基因芯片技術(shù)進(jìn)行數(shù)據(jù)分析。結(jié)果在支持細(xì)胞全基因組表達(dá)譜中,五子衍宗丸組與模型組比較共有1 203個差異表達(dá)基因,其中下調(diào)基因有697個,上調(diào)基因506個;正常組與模型組比較共有473個差異表達(dá)基因,其中上調(diào)基因168個,下調(diào)基因305個;與模型組相比,五子衍宗丸組和正常組共同下調(diào)的基因中RGD1305298、Trpt1、Dll3、Abi3和Ppp1r12b共5個基因表達(dá)差異明顯,共同上調(diào)的基因中Dusp7表達(dá)差異明顯,這些差異表達(dá)基因涉及到細(xì)胞周期、細(xì)胞凋亡、細(xì)胞代謝、細(xì)胞黏附等分子功能及Notch信號通路、細(xì)胞骨架調(diào)節(jié)、MAPK信號通路和緊密連接等通路。結(jié)論五子衍宗丸可能是通過維持支持細(xì)胞間緊密連接,抑制支持細(xì)胞凋亡,促進(jìn)支持細(xì)胞分化成熟來提高睪丸支持細(xì)胞熱應(yīng)激的能力。
[Abstract]:Aim to analyze the mechanism of Wizi Yanzong pill (WYPP) in improving the ability of testicular Sertoli cells to resist heat stress by using whole genome expression microarray data. Methods the primary cultured testicular Sertoli cells were divided into five groups: Wuzi Yanzong Pill group, normal group and model group. After 24 hours of culture with WZYZH solution and PBS, of equal volume, WZYZP group and model group were treated with water bath at 43 鈩，

本文編號：2320222