【摘要】：基于前期基因芯片結(jié)果,RT-PCR獲得栽培品種番茄M82和近緣野生種潘那利的一個(gè)WRKY基因的全長(zhǎng)cDNA序列,分別命名為SlWRKY41和SpWRKY41.序列分析表明,番茄WRKY41基因長(zhǎng)為1011bp,編碼336個(gè)氨基酸.該氨基酸序列含有5'-N端WRKYGQK核心結(jié)構(gòu)域和CX_7CX_(23)HX_1C鋅指結(jié)構(gòu),具有WRKY家族的典型結(jié)構(gòu)特征.同源分析表明,該氨基酸序列與多種植物的WRKY類蛋白具有較高的同源性,并且在普通栽培番茄品種M82和野生種潘那利中,只有5個(gè)氨基酸位點(diǎn)的差異.進(jìn)化樹分析表明,WRKY41在番茄中屬于第Ⅲ類WRKY蛋白,這類蛋白為植物所特有.表達(dá)分析結(jié)果表明,WRKY41在普通栽培番茄品種M82和野生種潘那利中,不僅在不同組織器官中的表達(dá)存在差異,而且在逆境(干旱和氧化逆境)和一些調(diào)節(jié)因子(SA、GA、乙烯)的處理下也有不同的表達(dá)模式.其在野生種潘那利中能夠迅速響應(yīng)相關(guān)調(diào)節(jié)因子(SA、GA、乙烯),推測(cè)WRKY41在番茄抗逆響應(yīng)過程中具有很重要的作用,Sp WRKY41可能是一個(gè)較好的改良普通栽培種抗逆性的候選基因.通過構(gòu)建超量表達(dá)載體,成功地將SpWRKY41轉(zhuǎn)化到番茄M82中,以期深入研究該基因的功能和提高番茄的抗逆性.
[Abstract]:Based on the results of previous gene microarray, RT-PCR obtained the full-length cDNA sequence of a WRKY gene of tomato M82 and a related wild species, Panali, named SlWRKY41 and SpWRKY41., respectively. Sequence analysis showed that the length of tomato WRKY41 gene was 1011 BP, encoding 336 amino acids. The amino acid sequence contains a 5- N-terminal WRKYGQK core domain and a CX_7CX_ (23) HX_1C zinc finger structure, which has the typical structural characteristics of the WRKY family. Homology analysis showed that the amino acid sequence had high homology with WRKY proteins of many plants, and there were only 5 amino acid loci in common cultivated tomato variety M82 and wild variety Panali. Phylogenetic tree analysis showed that WRKY41 belonged to class 鈪，

本文編號(hào)：2319967