【摘要】：土壤中的鉛污染存在分布范圍廣、持續(xù)時(shí)間長(zhǎng)、不易在物質(zhì)循環(huán)和能量交換中分解、易蓄積等特點(diǎn),給人類的生存環(huán)境和人類健康造成嚴(yán)重威脅。近年來(lái),植物修復(fù)技術(shù)以成本低,不引起二次污染的優(yōu)點(diǎn),受到各國(guó)科學(xué)家的關(guān)注。在已發(fā)現(xiàn)的鉛超富集植物中往往受到生物量小、生長(zhǎng)周期長(zhǎng)或地理環(huán)境等因素的限制,導(dǎo)致在土壤植物修復(fù)中得不到實(shí)際的應(yīng)用。隨著轉(zhuǎn)基因技術(shù)的的迅速發(fā)展,通過(guò)外源耐性基因在植物體內(nèi)的過(guò)量表達(dá)的方法,可以培育出生物量大、生長(zhǎng)周期短的超富集轉(zhuǎn)基因植物。根據(jù)以往的報(bào)道,關(guān)于耐鉛基因的甚少。本研究利用新一代高通量Illumina測(cè)序技術(shù)、以篩選出的鉛超富集植物黑心菊為研究對(duì)象,通過(guò)轉(zhuǎn)錄組測(cè)序,得到了大量的原始序列。對(duì)得到的高質(zhì)量序列進(jìn)行拼接、組裝和功能注釋,發(fā)掘其與耐鉛相關(guān)的基因,為土壤Pb污染植物修復(fù)提供更多的參考。同時(shí),通過(guò)RT-PCR法克隆了黑心菊的Rh NRAMP1基因并構(gòu)建了雙元克隆載體,為進(jìn)一步驗(yàn)證其基因功能和深入了解黑心菊耐鉛機(jī)制提供了基礎(chǔ)材料。
[Abstract]:Lead pollution in soil has the characteristics of wide distribution, long duration, not easy to decompose in material cycle and energy exchange, easy to accumulate, and so on, which poses a serious threat to human living environment and human health. In recent years, phytoremediation technology has attracted the attention of scientists all over the world because of its advantages of low cost and no secondary pollution. It is often limited by such factors as small biomass, long growth period or geographical environment in the discovered lead enrichment plants, which lead to no practical application in soil phytoremediation. With the rapid development of transgenic technology, super-enriched transgenic plants with large biomass and short growth cycle can be obtained by overexpression of exogenous tolerance genes in plants. According to previous reports, there are very few lead-tolerant genes. In this study, a new generation of high-throughput Illumina sequencing technology was used to select the lead super-enriched plant, chrysanthemum nigra, and a large number of primordial sequences were obtained by transcriptome sequencing. The high quality sequences were spliced, assembled and annotated, and the genes related to lead tolerance were discovered, which provided more references for phytoremediation of soil Pb contamination. At the same time, the Rh NRAMP1 gene was cloned by RT-PCR method and the binary cloning vector was constructed, which provided a basic material for further verification of its gene function and further understanding of the lead tolerance mechanism of the chrysanthemum nigra.
【學(xué)位授予單位】：貴州師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2016
【分類號(hào)】：Q943.2;X173

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 張彬;杜芳;;不同浸種方式對(duì)黑心菊種子萌發(fā)特性的影響[J];亞熱帶植物科學(xué);2012年04期

相關(guān)會(huì)議論文 前1條

1 劉倩;李芳;;園林綠化廢棄物堆肥在盆栽草花中的應(yīng)用研究[A];2011北京園林綠化與生物多樣性保護(hù)[C];2011年

相關(guān)重要報(bào)紙文章 前1條

1 河北省衡水市園林管理局 張素琴;如何讓綠化帶內(nèi)黑心菊達(dá)到優(yōu)良景觀效果[N];中國(guó)花卉報(bào);2014年

相關(guān)碩士學(xué)位論文 前2條

1 馬超;蜀葵、黑心菊對(duì)銅尾礦的耐性及銅污染環(huán)境的修復(fù)研究[D];江西財(cái)經(jīng)大學(xué);2015年

2 宋會(huì)焱;黑心菊耐鉛基因的發(fā)掘與克隆[D];貴州師范大學(xué);2016年

，

本文編號(hào)：2315264