【摘要】：為研究枇杷(Eriobotrya japonica Lindl.)脫水素基因(Ej DHN5)在枇杷抗低溫脅迫中的功能,將其在煙草中進(jìn)行過(guò)量表達(dá),其中表達(dá)水平比較高的兩個(gè)株系L24和L26被用來(lái)進(jìn)行功能研究。野生型和轉(zhuǎn)基因煙草苗分別用0、2、4和8μmol·L~(-1)的甲基紫精(Methyl viologen,自由基發(fā)生劑)處理并進(jìn)行光照培養(yǎng),結(jié)果顯示,4和8μmol·L~(-1)甲基紫精處理使野生型煙草苗存活率分別下降到73.6%和56.9%,光系統(tǒng)Ⅱ(PSⅡ)活性顯著降低,而轉(zhuǎn)基因苗的存活率仍維持在100%,PSⅡ活性明顯高于野生型。對(duì)六葉期煙草的葉盤(pán)進(jìn)行甲基紫精處理,野生型煙草葉盤(pán)的葉綠素含量顯著低于轉(zhuǎn)基因株系,而活性氧含量和MDA含量明顯高于轉(zhuǎn)基因株系。對(duì)野生型和轉(zhuǎn)基因煙草苗進(jìn)行低溫處理,轉(zhuǎn)基因株系生長(zhǎng)狀況明顯優(yōu)于野生型,活性氧含量低于野生型,膜質(zhì)過(guò)氧化程度輕于野生型。這些結(jié)果表明,過(guò)量表達(dá)EjDHN5能提高煙草的抗氧化脅迫和抗低溫脅迫能力,推測(cè)EjDHN5在提高枇杷抗低溫能力方面起著重要作用可能與其能提高抗氧化脅迫能力有關(guān)。
[Abstract]:Study on loquat (Eriobotrya japonica Lindl.) The function of dehydrin gene (Ej DHN5) in loquat resistance to low temperature stress was overexpressed in tobacco. Two lines L24 and L26 with higher expression level were used to study their function. Wild type and transgenic tobacco seedlings were treated with (Methyl viologen, free radical generator of 0 and 8 渭 mol L ~ (-1), respectively, and cultured under light. The survival rate of wild type tobacco seedlings decreased to 73.6% and 56.9%, respectively, and the activity of PS 鈪，

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