桂花DAD-1基因克隆與表達(dá)分析
發(fā)布時間：2018-11-01 20:40
【摘要】：以"柳葉金桂"桂花(Osmanthus fragrans‘Liuye Jingui’)為試材,采用TAIL-PCR技術(shù),克隆了"柳葉金桂"花瓣中DAD-1基因的全長序列,并分析了其在桂花不同組織部位及開花階段的時空表達(dá)模式,以期為研究該基因在桂花花瓣衰老過程中的功能提供參考依據(jù)。結(jié)果表明:DAD-1基因由560個堿基組成,包含一個351bp的完整開放閱讀框和一個3′-Poly A尾巴;DAD-1基因編碼的蛋白包含116個氨基酸,其結(jié)構(gòu)預(yù)測表明DAD-1蛋白屬于疏水性膜整合蛋白,通過3個α?螺旋構(gòu)成的跨膜區(qū)行使功能。半定量PCR結(jié)果表明,該基因在"柳葉金桂"的根、莖、葉、花等各組織器官中均有表達(dá),且在花苞、新葉等幼嫩組織器官中相對表達(dá)量較高,在老葉等成熟組織器官中相對表達(dá)量較低。實(shí)時熒光定量PCR結(jié)果表明,在"柳葉金桂"花瓣衰老過程中,DAD-1基因在鈴梗期開始顯著下調(diào)表達(dá)。
[Abstract]:The full-length sequence of DAD-1 gene in the petals of "Cinnamomum willow" was cloned by means of TAIL-PCR technique. The temporal and spatial expression patterns of the gene in different tissues and flowering stages of Osmanthus fragrans were analyzed in order to provide a reference for studying the function of the gene in the senescence process of Osmanthus osmanthus petals. The results showed that the DAD-1 gene was composed of 560 bases and contained a complete open reading frame of 351bp and a tail of 3'-Poly A. The protein encoded by DAD-1 gene contains 116 amino acids, and its structure prediction indicates that DAD-1 protein belongs to hydrophobic membrane integrin protein. The function of the transmembrane region formed by the helix. The results of semi-quantitative PCR showed that the gene was expressed in root, stem, leaf, flower and so on, and the relative expression was higher in young tissues such as bud and new leaf. The relative expression in mature tissues and organs such as old leaves was low. The results of real-time fluorescence quantitative PCR showed that the expression of DAD-1 gene began to down-regulate significantly during the senescence of the petals of "Cinnamomum willow".
【作者單位】： 湖北科技學(xué)院核技術(shù)與化學(xué)生物學(xué)院;孝感市農(nóng)業(yè)科學(xué)院蔬菜研究所;華中農(nóng)業(yè)大學(xué)園藝植物生物學(xué)教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：湖北省教育廳科學(xué)技術(shù)研究資助項(xiàng)目(Q20162805) 湖北科技學(xué)院博士啟動基金資助項(xiàng)目(BK1429)
【分類號】：S685.13
本文編號：2305041
[Abstract]:The full-length sequence of DAD-1 gene in the petals of "Cinnamomum willow" was cloned by means of TAIL-PCR technique. The temporal and spatial expression patterns of the gene in different tissues and flowering stages of Osmanthus fragrans were analyzed in order to provide a reference for studying the function of the gene in the senescence process of Osmanthus osmanthus petals. The results showed that the DAD-1 gene was composed of 560 bases and contained a complete open reading frame of 351bp and a tail of 3'-Poly A. The protein encoded by DAD-1 gene contains 116 amino acids, and its structure prediction indicates that DAD-1 protein belongs to hydrophobic membrane integrin protein. The function of the transmembrane region formed by the helix. The results of semi-quantitative PCR showed that the gene was expressed in root, stem, leaf, flower and so on, and the relative expression was higher in young tissues such as bud and new leaf. The relative expression in mature tissues and organs such as old leaves was low. The results of real-time fluorescence quantitative PCR showed that the expression of DAD-1 gene began to down-regulate significantly during the senescence of the petals of "Cinnamomum willow".
【作者單位】： 湖北科技學(xué)院核技術(shù)與化學(xué)生物學(xué)院;孝感市農(nóng)業(yè)科學(xué)院蔬菜研究所;華中農(nóng)業(yè)大學(xué)園藝植物生物學(xué)教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：湖北省教育廳科學(xué)技術(shù)研究資助項(xiàng)目(Q20162805) 湖北科技學(xué)院博士啟動基金資助項(xiàng)目(BK1429)
【分類號】：S685.13
【相似文獻(xiàn)】
相關(guān)期刊論文 前1條
1 韓永華,金危危,王小蘭,宋運(yùn)淳;水稻程序性細(xì)胞死亡抑制基因Dad-1在薏苡中的定位[J];武漢植物學(xué)研究;2003年06期
相關(guān)碩士學(xué)位論文 前1條
1 陸玲;桂花DAD-1同源基因片段的克隆及表達(dá)分析[D];華中農(nóng)業(yè)大學(xué);2009年
，本文編號：2305041
本文鏈接：http://sikaile.net/kejilunwen/jiyingongcheng/2305041.html
最近更新
教材專著
