【摘要】：以"柳葉金桂"桂花(Osmanthus fragrans‘Liuye Jingui’)為試材,采用TAIL-PCR技術,克隆了"柳葉金桂"花瓣中DAD-1基因的全長序列,并分析了其在桂花不同組織部位及開花階段的時空表達模式,以期為研究該基因在桂花花瓣衰老過程中的功能提供參考依據(jù)。結果表明:DAD-1基因由560個堿基組成,包含一個351bp的完整開放閱讀框和一個3′-Poly A尾巴;DAD-1基因編碼的蛋白包含116個氨基酸,其結構預測表明DAD-1蛋白屬于疏水性膜整合蛋白,通過3個α?螺旋構成的跨膜區(qū)行使功能。半定量PCR結果表明,該基因在"柳葉金桂"的根、莖、葉、花等各組織器官中均有表達,且在花苞、新葉等幼嫩組織器官中相對表達量較高,在老葉等成熟組織器官中相對表達量較低。實時熒光定量PCR結果表明,在"柳葉金桂"花瓣衰老過程中,DAD-1基因在鈴梗期開始顯著下調(diào)表達。
[Abstract]:The full-length sequence of DAD-1 gene in the petals of "Cinnamomum willow" was cloned by means of TAIL-PCR technique. The temporal and spatial expression patterns of the gene in different tissues and flowering stages of Osmanthus fragrans were analyzed in order to provide a reference for studying the function of the gene in the senescence process of Osmanthus osmanthus petals. The results showed that the DAD-1 gene was composed of 560 bases and contained a complete open reading frame of 351bp and a tail of 3'-Poly A. The protein encoded by DAD-1 gene contains 116 amino acids, and its structure prediction indicates that DAD-1 protein belongs to hydrophobic membrane integrin protein. The function of the transmembrane region formed by the helix. The results of semi-quantitative PCR showed that the gene was expressed in root, stem, leaf, flower and so on, and the relative expression was higher in young tissues such as bud and new leaf. The relative expression in mature tissues and organs such as old leaves was low. The results of real-time fluorescence quantitative PCR showed that the expression of DAD-1 gene began to down-regulate significantly during the senescence of the petals of "Cinnamomum willow".
【作者單位】： 湖北科技學院核技術與化學生物學院;孝感市農(nóng)業(yè)科學院蔬菜研究所;華中農(nóng)業(yè)大學園藝植物生物學教育部重點實驗室;
【基金】：湖北省教育廳科學技術研究資助項目(Q20162805) 湖北科技學院博士啟動基金資助項目(BK1429)
【分類號】：S685.13

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