利用高密度SNP遺傳圖譜定位小麥穗部性狀基因
[Abstract]:There is a close correlation among panicle traits, among which the number of grains per ear and 1000-grain weight are important components of yield. It is important to excavate the gene loci associated with panicle traits for molecular marker-assisted breeding and to explain gene effects. In this study, 173 F _ (8:9) lines of RIL population (Shannong 01-35 脳 Gaocheng 9411) were used as materials, the high-density genetic map was constructed by using 90k wheat SNP gene chip, DAr T chip technology and traditional molecular marker technology. QTL mapping of panicle related traits was carried out in 5 environments. An additive QTL, interpretation phenotype variation rate of 6.00 / 36.30g was detected on chromosome 1BX 4BX 5BN 6A to control 1000-grain weight, and the additive effect was all from Shannong 01-35, a large grain parent. Eight additive QTL, interpretations of phenotypic variation rates were detected, which controlled panicle length at 14.34% and 25.44%, respectively, and 3 controlled spikelets were controlled by grain number. The additive QTL, contribution rate of the male sterile spikelets was 8.70% and 37.70% respectively, and the additive QTL; of 4 total spikelets controlled the total spikelet number, and the additive QTL. of the spikelet density controlled 6% of the total spikelet number of male sterile spikelets, and the additive QTL, contribution rate of the sterile spikelet number was 8.70% and 37.70% respectively. By genotypic and environmental interaction analysis, 32 additive QTL, were detected to explain the phenotypic variation rate of 0.05% or 1.05%. In chromosome 4B, EX_C101685 RAC875_C27536 detected the controlled grain weight, panicle length, grain number per spike, fertile spikelet number, sterile spikelet number, total spikelet number and its contribution rate was 5.40% 37.70%. This locus was detected in many environments and was a stable principal QTL.. QTL. with controlled grain weight and total spikelet number was detected in the wPt-0959-TaGw2-CAPS interval of chromosome 6A. The results laid a foundation for molecular marker development, gene fine mapping and functional gene cloning of panicle traits.
【作者單位】: 山東農(nóng)業(yè)大學農(nóng)學院小麥品質(zhì)育種研究室/作物生物學國家重點實驗室;
【基金】:山東省自然科學基金項目(2015ZRB01179,ZR2013CM004) 山東省種質(zhì)資源創(chuàng)制課題資助~~
【分類號】:S512.1
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