【摘要】：ABI5(abscisic acid-insensitive 5)蛋白是一個響應ABA信號的堿性亮氨酸拉鏈類(basic leucine zipper,b ZIP)轉錄因子。揭示了水曲柳FmABI5在非生物脅迫下的表達特征,為該基因在水曲柳中代謝調控功能的研究奠定基礎。獲得了水曲柳ABI5基因的全長,命名為FmABI5,應用生物信息學軟件分析了水曲柳FmABI5基因的分子結構特征,利用PEG、低溫(4℃)及鹽(NaCl)進行非生物脅迫處理,利用脫落酸(ABA)和赤霉素(GA3)進行信號誘導,分析FmABI5的表達特征。生物信息學分析表明該基因全長1 455 bp,含有完整的開放閱讀框,編碼484個氨基酸。FmABI5為不穩(wěn)定類親水性蛋白,不存在信號肽,具有跨膜能力,α-螺旋、延伸鏈、無規(guī)則卷曲分布于整個蛋白。分子進化分析結果表明,水曲柳FmABI5基因與芝麻的遺傳距離較近,說明其親緣關系較近;與馬鈴薯、潘那利番茄、水茄與甜椒的遺傳距離較遠,說明其親緣關系較遠。非生物脅迫結果表明,FmABI5基因表達水平隨非生物脅迫處理時間不同而上下波動,但在處理6、48和72 h后,FmABI5基因對3種非生物脅迫處理都上調表達,說明FmABI5基因對非生物脅迫具有響應。信號誘導結果表明,外源的ABA與GA共同調節(jié)了FmABI5基因的表達。
[Abstract]:ABI5 (abscisic acid-insensitive 5) protein is a basic leucine zipper (basic leucine zipper,b ZIP transcription factor that responds to ABA signal. The expression characteristics of FmABI5 in Fraxinus mandshurica under abiotic stress were revealed, which laid a foundation for the study of metabolic regulation of the gene in Fraxinus mandshurica. The full length of ABI5 gene of Fraxinus mandshurica was obtained. The molecular structure of FmABI5 gene of Fraxinus mandshurica was analyzed by using bioinformatics software FmABI5, and abiotic stress was carried out by using PEG, low temperature (4 鈩，

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