【摘要】：全蝕病是小麥上一種重要的土傳病害。選育和種植抗病品種是防治小麥全蝕病的根本途徑,抗病基因研究是抗病育種的基礎(chǔ)性工作。根據(jù)基因TaWIR1b(Accession no.M94959.1)的全長(zhǎng)序列設(shè)計(jì)引物擴(kuò)增‘新農(nóng)19’的cDNA,獲得了完整ORF,編碼85個(gè)氨基酸殘基,比對(duì)后發(fā)現(xiàn)與TaWIR1b序列同源性達(dá)100%。根據(jù)獲得的TaWIR1b基因全長(zhǎng)序列設(shè)計(jì)定量引物,分析TaWIR1b在全蝕菌脅迫條件下不同互作模式的表達(dá)特征。結(jié)果表明接種全蝕病菌后抗病小麥品種‘新農(nóng)19’中TaWIR1b基因被誘導(dǎo)表達(dá),接菌后3d達(dá)到峰值143.97,感病品種‘新麥19’中峰值出現(xiàn)在接菌后8d,表達(dá)量?jī)H為對(duì)照的4.22倍,提示該基因可能參與小麥對(duì)全蝕病的抗病過程。
[Abstract]:Total erosion is an important soil-borne disease in wheat. Breeding and planting of disease-resistant varieties is the fundamental way to control the total corrosion of wheat, and the research of disease-resistant genes is the basic work of disease-resistant breeding. According to the full-length sequence of the gene TaWIR1b (Accession no.M94959.1), a primer was designed to amplify the cDNA, of 'Xinnong 19'. The complete ORF, encoding 85 amino acid residues was obtained, and the homology with TaWIR1b sequence was 100%. According to the obtained full-length sequence of TaWIR1b gene, quantitative primers were designed to analyze the expression characteristics of different interaction patterns of TaWIR1b under total corrosion stress. The results showed that the expression of TaWIR1b gene in resistant wheat variety 'Xinnong 19' was induced after inoculation, and reached a peak value of 143.97 at 3 days after inoculation. The peak value in susceptible variety 'Xinmai 19' appeared at 8 days after inoculation, and the expression level was only 4.22 times as much as that of the control. These results suggest that the gene may be involved in the disease resistance of wheat to total corrosion.
【作者單位】： 河南省農(nóng)業(yè)科學(xué)院植物保護(hù)研究所 農(nóng)業(yè)部華北南部作物有害生物綜合治理重點(diǎn)實(shí)驗(yàn)室;
【基金】：河南省小麥產(chǎn)業(yè)技術(shù)體系(S2010-01-05) 河南省基礎(chǔ)與前沿技術(shù)研究計(jì)劃項(xiàng)目(142300413221) 河南省農(nóng)業(yè)科學(xué)院自主創(chuàng)新基金
【分類號(hào)】：S435.121.49
，

本文編號(hào)：2260940