蝴蝶蘭C類花器官發(fā)育基因PhAG1a的克隆及表達分析
發(fā)布時間：2018-10-04 20:34
【摘要】：為研究MADS-box基因在花器官發(fā)育中的功能,進一步理解蘭科植物花器官發(fā)育的分子調(diào)控機理。利用RT-PCR和RACE技術(shù)從蝴蝶蘭中克隆了一個MADS-box基因PhAG1a(GenBank登錄號為KY399811),并利用實時熒光定量方法分析其表達特性。序列分析表明,該基因的cDNA全長為1 107 bp,含完整的開放閱讀框,可編碼248個氨基酸,屬于C功能AGAMOUSE家族基因,與蝴蝶蘭屬的PhalAG1和PeMADS1基因關(guān)系最近;表達模式分析表明,PhAG1a基因在營養(yǎng)器官中不表達,只在蕊柱和子房中表達。在5類突變體花器官中,PhAG1a基因在退化雄蕊變異為側(cè)瓣、側(cè)瓣退化與蕊柱合生和側(cè)萼片變異為唇瓣等3類突變體花器官中,PhAG1a基因的表達沒有變化,在側(cè)瓣變異為唇瓣和側(cè)瓣頂端長出花藥的突變體花器官中,PhAG1a基因在蕊柱中的表達水平顯著降低。推測該基因在決定蝴蝶蘭合蕊柱的發(fā)育中起重要的調(diào)控作用。
[Abstract]:In order to study the function of MADS-box gene in floral organ development, the molecular regulation mechanism of floral organ development in orchid plants was further understood. A MADS-box gene PhAG1a (GenBank accession number KY399811) was cloned from Phalaenopsis by RT-PCR and RACE techniques, and its expression characteristics were analyzed by real-time fluorescence quantitative method. Sequence analysis showed that the total length of cDNA was 1 107 bp, which contained a complete open reading frame, encoding 248 amino acids, belonging to the C-functional AGAMOUSE family, which had the closest relationship with the PhalAG1 and PeMADS1 genes of Phalaenopsis. The expression pattern analysis showed that the PhAG1a gene was not expressed in vegetative organs, but only in stamen and ovary. In floral organs of five mutants, the expression of PhAG1a gene was not changed in floral organs of three types of mutants, such as staminodes, lateral petal and stamen column, and lateral sepals, and so on, and the expression of PhAG1a gene was not changed in floral organs of the five mutants, and there was no change in the expression of PhAG1a gene in floral organs. The expression level of PhAG1a gene in the floral organs of the mutant which mutated to lip and the tip of the lateral valve was significantly decreased in the stamen column. It is speculated that this gene plays an important role in determining the development of syphalopsis in Phalaenopsis.
【作者單位】： 鄭州師范學(xué)院生物工程研究所;鄭州師范學(xué)院生命科學(xué)學(xué)院;
【基金】：河南省科技攻關(guān)項目(No.162102110073)
【分類號】：Q943.2;S682.31
，
本文編號：2251789
[Abstract]:In order to study the function of MADS-box gene in floral organ development, the molecular regulation mechanism of floral organ development in orchid plants was further understood. A MADS-box gene PhAG1a (GenBank accession number KY399811) was cloned from Phalaenopsis by RT-PCR and RACE techniques, and its expression characteristics were analyzed by real-time fluorescence quantitative method. Sequence analysis showed that the total length of cDNA was 1 107 bp, which contained a complete open reading frame, encoding 248 amino acids, belonging to the C-functional AGAMOUSE family, which had the closest relationship with the PhalAG1 and PeMADS1 genes of Phalaenopsis. The expression pattern analysis showed that the PhAG1a gene was not expressed in vegetative organs, but only in stamen and ovary. In floral organs of five mutants, the expression of PhAG1a gene was not changed in floral organs of three types of mutants, such as staminodes, lateral petal and stamen column, and lateral sepals, and so on, and the expression of PhAG1a gene was not changed in floral organs of the five mutants, and there was no change in the expression of PhAG1a gene in floral organs. The expression level of PhAG1a gene in the floral organs of the mutant which mutated to lip and the tip of the lateral valve was significantly decreased in the stamen column. It is speculated that this gene plays an important role in determining the development of syphalopsis in Phalaenopsis.
【作者單位】： 鄭州師范學(xué)院生物工程研究所;鄭州師范學(xué)院生命科學(xué)學(xué)院;
【基金】：河南省科技攻關(guān)項目(No.162102110073)
【分類號】：Q943.2;S682.31
，
本文編號：2251789
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