【摘要】：[目的]水稻葉片作為重要的光合器官,是作物產(chǎn)量的形成基礎(chǔ)。葉色突變體不僅可以作為育種標(biāo)記,還是研究葉綠體發(fā)育和培育高光效水稻品種的先決條件之一。[方法]從秈稻品種‘9311’的~(60)Co-γ射線輻射誘變突變體庫(kù)中篩選獲得了1個(gè)水稻黃葉突變體yl。利用yl/‘寧粳4號(hào)’雜交的F2群體進(jìn)行基因定位。[結(jié)果]與野生型相比,yl突變體的幼葉表現(xiàn)出明顯的黃化,葉綠素a、葉綠素b、類胡蘿卜素的含量下降。突變體葉綠體中類囊體片層結(jié)構(gòu)排列松散。與野生型相比,yl突變體的每穗粒數(shù)、結(jié)實(shí)率和千粒質(zhì)量下降。該性狀受1對(duì)隱性核基因控制。該基因初步定位于第9染色體長(zhǎng)臂上,進(jìn)一步開發(fā)分子標(biāo)記把定位區(qū)間縮小在14.5 kb內(nèi),該區(qū)域包含3個(gè)候選基因。經(jīng)測(cè)序比對(duì)分析發(fā)現(xiàn),yl突變體的第3個(gè)ORF的第5外顯子發(fā)生堿基突變(2131CTA→G),從而造成蛋白第233位氨基酸發(fā)生改變并導(dǎo)致翻譯提前終止,形成了1個(gè)包含原有232個(gè)氨基酸和16個(gè)新形成氨基酸殘基的蛋白,該基因與已報(bào)道的水稻黃葉基因YLC1是等位基因。[結(jié)論]本研究明確了YLC1參與葉綠素的合成,為葉綠體色素合成和葉綠體發(fā)育的研究提供了基礎(chǔ)。
[Abstract]:Objective: as an important photosynthetic organ, rice leaf is the basis of crop yield. Leaf color mutants can be used not only as breeding markers, but also as a prerequisite for chloroplast development and breeding of high light efficiency rice varieties. [methods] A rice yellow leaf mutant yl. was obtained from the mutant library of ~ (60) Co- 緯 -ray radiation induced by indica rice cultivar Zeng9311'. The F 2 population of yl/' Ningjing 4 'crossing was used to localize the gene. [results] compared with the wild type, the young leaves of the mutant showed obvious yellowing, and the contents of chlorophyll a, chlorophyll b and carotenoid decreased. Thylakoid lamellae in mutant chloroplasts is loosely arranged. Compared with wild type, the number of kernels per panicle, the seed setting rate and the weight of 1000 kernels decreased. This trait is controlled by a pair of recessive nuclear genes. The gene was preliminarily located on the long arm of chromosome 9 and further developed molecular markers to narrow the localization range to 14. 5 kb. The region contains three candidate genes. Sequencing analysis revealed that the 2131CTA G), of the third ORF of the mutants had a base mutation (2131CTA G), which resulted in the change of amino acid at the 233rd position of the protein and the early termination of translation. A protein containing 232 amino acids and 16 newly formed amino acid residues was formed. The gene is allelic to the reported rice yellow leaf gene YLC1. [conclusion] YLC1 is involved in the synthesis of chlorophyll, which provides a basis for the study of chloroplast pigment synthesis and chloroplast development.
【作者單位】： 南京農(nóng)業(yè)大學(xué)作物遺傳與種質(zhì)創(chuàng)新國(guó)家重點(diǎn)實(shí)驗(yàn)室/江蘇省現(xiàn)代作物生產(chǎn)協(xié)同創(chuàng)新中心;
【基金】：國(guó)家科技支撐計(jì)劃項(xiàng)目(2015BAD01B02-7)
【分類號(hào)】：S511;Q943.2
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本文編號(hào)：2247137