【摘要】：本試驗(yàn)選用44日齡、平均體重8.94 kg的DLY三元雜交斷奶仔豬60頭,隨機(jī)分為5個(gè)處理組,每個(gè)處理3個(gè)重復(fù),每個(gè)重復(fù)4頭豬。第一組為對(duì)照組,飼喂基礎(chǔ)日糧;第二、第三、第四和第五組為試驗(yàn)組,分別在基礎(chǔ)日糧中添加抗生素(桿菌肽鋅+硫酸粘桿菌素)及0.05%、0.10%、0.15%的白術(shù)多糖(PAM),正式期為30 d。30 d后頸靜脈采取血液樣品,并應(yīng)用SYBR Green I熒光染料實(shí)時(shí)定量PCR方法進(jìn)行白細(xì)胞介素-1β(IL-1β)基因mRNA的定量,研究白術(shù)多糖對(duì)斷奶仔豬免疫功能的影響,為白術(shù)多糖的深入研究提供科學(xué)依據(jù)。結(jié)果表明:IL-β基因的相對(duì)表達(dá)量,各處理組間差異不顯著(P0.05),但白術(shù)多糖處理組均比對(duì)照組和抗生素組高,其中以0.1%PAM組最高,抗生素組、0.05%PAM組次之,對(duì)照組和0.15%PAM處理組的最低。0.1%PAM處理組與對(duì)照組和抗生素組相比,IL-β基因相對(duì)表達(dá)量分別提高34.21%和27.5%;0.1%PAM處理組與0.05%PAM處理組和0.15%PAM處理組相比,IL-β基因相對(duì)表達(dá)量分別提高27.5%和34.21%。0.05%PAM處理組與對(duì)照組和抗生素組相比,IL-β基因相對(duì)表達(dá)量分別提高5.2%和0%;0.15%PAM處理組與對(duì)照組相比,IL-β基因相對(duì)表達(dá)量提高0%;0.15%PAM處理組與抗生素組相比,IL-β基因相對(duì)表達(dá)量降低5%。
[Abstract]:In this experiment, 60 DLY weaned piglets with average weight of 8.94 kg and 44 days old were randomly divided into 5 treatment groups, 3 replicates per treatment and 4 piglets per replica. The first group was the control group, fed the basic diet, and the second, third, fourth and fifth groups were the experimental groups. Blood samples were taken from jugular vein after 30 days after adding antibiotics (Bacteriopin zinc sulfate) and 0.05% Atractylodes macrocephala polysaccharides (PAM),) in basal diet for 30 d, respectively, and 0.15% of Atractylodes macrocephala polysaccharides (PAM),) were added to the basal diet for 30 days. The effect of Atractylodes macrocephala polysaccharides on the immune function of weaned piglets was studied by using real-time quantitative PCR method of SYBR Green I fluorescent dye to quantify the mRNA of IL-1 尾 gene, which provides a scientific basis for the further study of Atractylodes macrocephala polysaccharides in weaning piglets. The results showed that there was no significant difference in the relative expression of IL-尾 gene among different treatment groups (P0.05), but the Atractylodes macrocephala polysaccharide treatment group was higher than the control group and the antibiotic group, the highest in the 0.1%PAM group, followed by 0.05 PAM group in the antibiotic group. The relative expression of IL- 尾 gene increased by 34.21% in the control group and 27.50.1% in the 0.15%PAM treatment group compared with the control group and the antibiotic treatment group. The relative expression of the IL- 尾 gene in the PAM-treated group was increased by 27.5% compared with the 0.05%PAM treatment group and the 0.15%PAM treatment group, and the relative expression of IL- 尾 gene in the PAM-treated group was increased by 27.5% compared with the 0.05%PAM treatment group and the 0.15%PAM treatment group. Compared with the control group and the antibiotic group, the relative expression of IL- 尾 gene in the 34.21%.0.05%PAM treatment group was increased by 5.2%, and the relative expression level of the IL- 尾 gene in the 0 ~ (15) PAM group was increased by 0% compared with the control group. The relative expression of IL- 尾 gene in the 34.21%.0.05%PAM treatment group was decreased by 5% compared with the antibiotic group.
【作者單位】： 芒市科技局科協(xié);云南農(nóng)業(yè)大學(xué)動(dòng)物科學(xué)學(xué)院省動(dòng)物營(yíng)養(yǎng)與飼料科學(xué)重點(diǎn)實(shí)驗(yàn)室;濰坊六和飼料有限公司臨朐分公司;芒市畜牧局;德宏州畜牧局;德宏州科技局科協(xié);
【基金】：云南省運(yùn)用基礎(chǔ)研究面上基金項(xiàng)目(2007C0056M)
【分類號(hào)】：S828.5

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