發(fā)布時(shí)間：2018-09-12 20:17

【摘要】：胚胎發(fā)育晚期豐富蛋白(LEA)是一個(gè)小的高親水性的蛋白家族,該蛋白家族在逆境脅迫下大量積累,保護(hù)植物免受逆境脅迫。LEA蛋白可分為7組,其中重復(fù)的11-氨基酸基序是第3組LEA蛋白的特征。為深入分析第3組LEA蛋白在小麥響應(yīng)逆境脅迫中的作用機(jī)制,利用芯片技術(shù)從小麥表達(dá)譜中篩選出一個(gè)滲透脅迫誘導(dǎo)表達(dá)的第3組LEA蛋白基因TaLEAsm,然后根據(jù)該基因序列設(shè)計(jì)引物篩選石麥15的BAC文庫,獲得1個(gè)含有該基因的BAC單克隆,以該BAC單克隆質(zhì)粒為模板,通過BAC延伸測序克隆了TaLEAsm基因及其啟動(dòng)子序列,并對TaLEAsm序列特征、表達(dá)模式和啟動(dòng)子功能進(jìn)行了初步分析。結(jié)果表明,TaLEAsm基因序列僅含有1個(gè)105bp的內(nèi)含子,其開放讀碼框長675bp,編碼224個(gè)氨基酸。TaLEAsm含有10個(gè)11-氨基酸重復(fù)序列,屬于第3組LEA蛋白。低溫、高鹽和滲透脅迫均誘導(dǎo)TaLEAsm基因上調(diào)表達(dá),但在根和葉中表達(dá)模式不同。在TaLEAsm基因起始密碼子上游1 500bp序列中,預(yù)測含有14個(gè)逆境響應(yīng)順式元件。在擬南芥中,TaLEAsm基因啟動(dòng)子能夠啟動(dòng)GUS基因表達(dá),滲透脅迫誘導(dǎo)GUS基因明顯上調(diào)表達(dá)。以上結(jié)果表明,TaLEAsm為小麥脫水響應(yīng)基因,其啟動(dòng)子為滲透脅迫誘導(dǎo)啟動(dòng)子。
[Abstract]:Late embryonic development rich protein (LEA) is a small high hydrophilic protein family, which accumulates under stress, and can be divided into 7 groups to protect plants from stress. The repeated 11-amino acid motifs are the characteristics of the third group of LEA proteins. In order to analyze the mechanism of the third group of LEA protein in wheat response to stress, A third group of LEA protein gene TaLEAsm, was screened from wheat expression profile by microarray technique. According to the gene sequence, primers were designed to screen the BAC library of Shimai 15. A BAC monoclonal containing the gene was obtained. Using the BAC monoclonal plasmid as template, the TaLEAsm gene and its promoter sequence were cloned by BAC extension sequencing, and the characteristics, expression patterns and promoter functions of TaLEAsm sequence were preliminarily analyzed. The results showed that the Tale Asm gene contained only one intron of 105bp, and its open reading frame was 675 BP, encoding 224 amino acids. Tale Asm contained 10 11 amino acid repeats, belonging to the third group of LEA proteins. Low temperature, high salt and osmotic stress all induced the up-regulation of TaLEAsm gene expression, but the expression patterns were different in root and leaf. In the upstream 1 500bp sequence of the start codon of the TaLEAsm gene, 14 adduct-response elements were predicted. In Arabidopsis thaliana (Arabidopsis thaliana), the promoter of Tale Asm gene could activate the expression of GUS gene, and the expression of GUS gene was up-regulated by osmotic stress. The results indicated that Tale Asm was a dehydration response gene of wheat and its promoter was osmotic stress induced promoter.
【作者單位】： 石家莊市農(nóng)林科學(xué)研究院;
【基金】：國家重點(diǎn)研發(fā)計(jì)劃項(xiàng)目(2016YFD0101802) 國家公益性行業(yè)(農(nóng)業(yè))科研專項(xiàng)(201203012) 河北省科技支撐計(jì)劃項(xiàng)目(16226320D)
【分類號】：S512.1

【參考文獻(xiàn)】

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