江漢油田區(qū)典型農(nóng)田土壤烴類降解微生物功能基因bssA的遺傳多樣性研究
發(fā)布時間：2018-09-04 16:30
【摘要】：油田區(qū)土壤易受烴類物質(zhì)影響并可能富集了特異的石油烴降解微生物類群。針對江漢油田區(qū)5個不同油井口附近的典型旱地農(nóng)田土壤,采用石油烴(Petroleum hydrocarbons,PHs)中苯系物代謝的關(guān)鍵功能基因-bssA(苯甲基琥珀酸合成酶基因)作為分子標識物,通過克隆文庫結(jié)合末端限制性片段長度多樣性(Terminal-restriction fragment length polymorphism,T-RFLP)的方法,研究該油田區(qū)土壤含有bssA基因的烴類降解微生物群落結(jié)構(gòu),并探討其環(huán)境驅(qū)動機制。結(jié)果表明,土壤中PAHs含量在0.21~2.01mg kg~(-1)之間,石油烴污染程度較低。T-RFLP的分析表明不同土壤樣品中的bssA基因多樣性差異明顯,PAHs(Polycyclic Aromatic Hydrocarbons,多環(huán)芳烴)含量最高土壤中bssA基因多樣性最高,其優(yōu)勢bssA基因類群與硫酸鹽還原菌或地桿菌有較近的親緣關(guān)系。冗余分析進一步表明,土壤硝態(tài)氮、有效磷、PAHs含量均是影響bssA基因多樣性的重要因子。這些結(jié)果表明:江漢油田區(qū)典型農(nóng)田土壤中含有bssA基因的主要類群為β-變形菌和δ-變形菌,并與地桿菌屬(Geobacter)、索氏菌屬(Thauera)和固氮菌屬(Azoarcus)具有較近的系統(tǒng)發(fā)育親緣關(guān)系。這些微生物可能通過硝酸鹽、硫酸鹽及鐵還原代謝過程降解土壤PAHs。
[Abstract]:The soil in oilfield area is easy to be affected by hydrocarbon and may enrich the specific microbial community of petroleum hydrocarbon degradation. In this paper, the key functional gene of benzene metabolism in petroleum hydrocarbon (Petroleum hydrocarbons,PHs), -bssA (phenylmethyl succinate synthase gene), was used as molecular marker for typical dryland farmland soil near five different well orifices in Jianghan Oilfield. The community structure of hydrocarbon-degrading microorganism containing bssA gene in soil of the oilfield area was studied by means of cloning library combined with terminal restriction fragment length diversity (Terminal-restriction fragment length polymorphism,T-RFLP) and its environmental driving mechanism was discussed. The results showed that the content of PAHs in soil was between 0.21~2.01mg kg~ (1) and the degree of petroleum hydrocarbon pollution was lower. T-RFLP analysis showed that the diversity of bssA gene in different soil samples was significantly different from that of PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons (PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons). The dominant bssA gene group was closely related to sulfate reducing bacteria or Dibacillus. Redundancy analysis further showed that the contents of nitrate nitrogen and available phosphorus in soil were important factors affecting the diversity of bssA gene. The results showed that the main groups of bssA gene in typical farmland soil of Jianghan Oilfield were 尾 -Proteus and 未 -Proteus, and had close phylogenetic relationship with (Thauera) of (Geobacter), and (Azoarcus) of Azotobacter. These microorganisms may degrade soil PAHs. through nitrate, sulfate and iron reduction metabolism.
【作者單位】： 西南大學(xué)資源環(huán)境學(xué)院;
【基金】：國家自然科學(xué)基金項目(41371477) 中央高校專項基金項目(XDJK2014B047)資助~~
【分類號】：X172;X53
，
本文編號：2222723
[Abstract]:The soil in oilfield area is easy to be affected by hydrocarbon and may enrich the specific microbial community of petroleum hydrocarbon degradation. In this paper, the key functional gene of benzene metabolism in petroleum hydrocarbon (Petroleum hydrocarbons,PHs), -bssA (phenylmethyl succinate synthase gene), was used as molecular marker for typical dryland farmland soil near five different well orifices in Jianghan Oilfield. The community structure of hydrocarbon-degrading microorganism containing bssA gene in soil of the oilfield area was studied by means of cloning library combined with terminal restriction fragment length diversity (Terminal-restriction fragment length polymorphism,T-RFLP) and its environmental driving mechanism was discussed. The results showed that the content of PAHs in soil was between 0.21~2.01mg kg~ (1) and the degree of petroleum hydrocarbon pollution was lower. T-RFLP analysis showed that the diversity of bssA gene in different soil samples was significantly different from that of PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons (PAHs (Polycyclic Aromatic Hydrocarbons, polycyclic aromatic hydrocarbons). The dominant bssA gene group was closely related to sulfate reducing bacteria or Dibacillus. Redundancy analysis further showed that the contents of nitrate nitrogen and available phosphorus in soil were important factors affecting the diversity of bssA gene. The results showed that the main groups of bssA gene in typical farmland soil of Jianghan Oilfield were 尾 -Proteus and 未 -Proteus, and had close phylogenetic relationship with (Thauera) of (Geobacter), and (Azoarcus) of Azotobacter. These microorganisms may degrade soil PAHs. through nitrate, sulfate and iron reduction metabolism.
【作者單位】： 西南大學(xué)資源環(huán)境學(xué)院;
【基金】：國家自然科學(xué)基金項目(41371477) 中央高校專項基金項目(XDJK2014B047)資助~~
【分類號】：X172;X53
，
本文編號：2222723
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