【摘要】：為了分析擬南芥脫落酸響應(yīng)蛋白R(shí)ING-V1基因(ABRv1)的功能,構(gòu)建了高表達(dá)載體pBI121-ABRv1,經(jīng)農(nóng)桿菌轉(zhuǎn)化,花序浸染后得到T0代轉(zhuǎn)基因種子,并經(jīng)卡那霉素板篩選得到轉(zhuǎn)基因苗,再經(jīng)過兩代的篩選獲取了純合的高表達(dá)株系A(chǔ)BRv1.通過DNA及RNA水平鑒定了ABRv1基因的T-DNA插入突變體abrv1.對(duì)突變體、高表達(dá)轉(zhuǎn)基因株系及野生型進(jìn)行ABA誘導(dǎo)處理,突變體的萌發(fā)率不足10%,野生型萌發(fā)率為40%,而過表達(dá)株系萌發(fā)率為67%;突變體株系氣孔幾乎全部關(guān)閉,過表達(dá)株系氣孔關(guān)閉不明顯,大小是突變體株系的2~3倍.結(jié)果表明ABRv1基因在擬南芥的ABA信號(hào)應(yīng)答響應(yīng)中可能起負(fù)調(diào)控作用.
[Abstract]:In order to analyze the function of abscisic acid responsive protein RING-V1 gene (ABRv1) in Arabidopsis thaliana, a high expression vector pBI121-ABRv1 was constructed. Transgenic seeds of T 0 generation were obtained by Agrobacterium tumefaciens transformation, inflorescence soaking, and transgenic seedlings were screened by kanamycin plate. After two generations of screening, the homozygous high expression line ABRv1 was obtained. The T-DNA insertion mutant abrv1 of ABRv1 gene was identified by DNA and RNA levels. The germinating rate of mutant, high expression transgenic line and wild type was less than 10%, the germination rate of wild type was 40 and that of over-expressed line was 67%, and the stomata of mutant were almost all closed. The stomatal closure of the overexpressed lines was not obvious, and the size of the overexpressed lines was 2 ~ 3 times as large as that of the mutant lines. The results suggest that ABRv1 gene may play a negative role in the response to ABA signaling in Arabidopsis thaliana.
【作者單位】： 四川大學(xué)生命科學(xué)學(xué)院生物資源與生態(tài)環(huán)境教育部重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金(31171586) 973計(jì)劃(2015CB755702)
【分類號(hào)】：Q943.2
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本文編號(hào)：2195187