【摘要】：3-磷酸甘油脫氫酶(GPDH)是一種在磷酸甘油酯的代謝過程中起重要調(diào)節(jié)作用的關(guān)鍵酶,研究植物中3-磷酸甘油脫氫酶基因家族的進化歷史及其多樣性對進一步探索其作用機制具有重要意義.以20個已報道的GPDH基因作為查詢序列,與19種代表性的植物物種的基因組分別進行BLASTP和t BLASTN比對以及隱馬爾科夫模型搜索,獲得基因家族新成員,構(gòu)建系統(tǒng)發(fā)育樹,并對其進行基因結(jié)構(gòu)、蛋白模體、功能差異性以及適應(yīng)性進化分析.預(yù)測得到了75個新的GPDH基因家族成員,系統(tǒng)發(fā)育分析表明,植物中的GPDH基因?qū)儆?個單系類群(命名為族群I、族群II和族群III),并且在進化過程中主要分為兩個進化枝,說明GPDH基因家族源自兩個GPDH祖先基因;功能差異性分析表明,氨基酸特異性位點的選擇性約束作用于不同族群的GPDH基因上,并且導(dǎo)致了不同族群多樣化之后的特異性進化,同時,在族群I/III和族群II/III中也發(fā)現(xiàn)了Type-II功能差異性,表明氨基酸的理化性質(zhì)也發(fā)生了本質(zhì)的改變;適應(yīng)性進化分析以及ka/ks比值分析表明,GPDH基因家族在物種特異性復(fù)制之后的進化過程中受到了純化選擇作用.因此,GPDH基因家族在其進化過程中受到不同的選擇壓力而出現(xiàn)了不同的進化方向,形成了多樣化的族群.此外,對該基因家族進行的系統(tǒng)的分子進化探索為植物中GPDH基因家族的進一步生物化學(xué)和遺傳方面的研究以及基因組分析提供了數(shù)據(jù)基礎(chǔ),在GPDH基因家族分析中的結(jié)果與發(fā)現(xiàn)也為研究其他基因家族提供了有力的幫助.
[Abstract]:Glycerol 3-phosphate dehydrogenase (GPDH) is a key enzyme that plays an important role in the metabolism of glycerol phosphate. It is important to study the evolutionary history and diversity of glycerol 3-phosphate dehydrogenase gene family in plants. Using 20 reported GPDH genes as query sequences, the genome of 19 representative plant species was compared with BLASTP and t BLASTN and hidden Markov model search. New members of gene family were obtained and phylogenetic tree was constructed. Gene structure, protein motifs, functional differences and adaptive evolution were analyzed. Phylogenetic analysis showed that the GPDH gene in plants belonged to three monoclines (population I, population II and population III), and they were mainly divided into two evolutionary branches during evolution. The functional difference analysis showed that the selective restriction of amino acid specific sites acted on the GPDH gene of different populations and led to the specific evolution of different populations after diversification. At the same time, the functional differences of Type-II were also found in I/III and II/III, indicating that the physical and chemical properties of amino acids had also changed substantially. Adaptive evolution analysis and ka/ks ratio analysis showed that the GPDH gene family was purified and selected during the evolution of species specific replication. As a result, the GPDH gene family is under different selection pressures in its evolution process, resulting in different evolution directions, forming a diverse population. In addition, the systematic molecular evolution of the GPDH gene family provides a data basis for further biochemical and genetic studies and genomic analysis of the GPDH gene family in plants. The results and findings in GPDH gene family analysis also provide powerful help for the study of other gene families.
【作者單位】： 四川大學(xué)生命科學(xué)學(xué)院微生物與代謝工程四川省重點實驗室;
【基金】：國家自然科學(xué)基金項目(31272659,31171447) “十二五”支撐計劃項目(2013BAD10B01,2014BAD02B02) 四川省科技廳項目(2013GZ0058,2014GZX0005,2014JCPT005)資助~~
【分類號】：Q943.2
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本文編號：2188257