【摘要】：Pita是一個(gè)對(duì)稻瘟病生理小種具有廣譜抗性的基因,通過對(duì)Pita抗病等位基因與感病等位基因編碼區(qū)進(jìn)行PCR擴(kuò)增、測(cè)序與序列比對(duì),在編碼區(qū)的+2 001 bp即內(nèi)含子中鑒定到一處特異性編碼序列(抗、感病等位基因分別為GCC和CTAT),針對(duì)該目標(biāo)位點(diǎn),本研究利用兩對(duì)交叉引物PCR(PCR with confronting two-pair primers,PCR-CTPP)方法開發(fā)出一套鑒定該目標(biāo)位點(diǎn)的共顯性標(biāo)記用于鑒定Pita等位基因類型,結(jié)果表明該標(biāo)記特異性強(qiáng)、穩(wěn)定、結(jié)果準(zhǔn)確、耗時(shí)少、成本低廉,可廣泛應(yīng)用于分子標(biāo)記輔助選擇育種或水稻資源中對(duì)Pita基因型鑒定、篩選的應(yīng)用。
[Abstract]:Pita is a broad-spectrum resistance gene to rice blast physiological races. The coding region of Pita resistance allele and susceptible allele was amplified by PCR, sequenced and sequenced. A specific coding sequence was identified in the 2 001 BP intron of the coding region. The anti- and susceptible alleles were GCC and CTAT), for the target site, respectively. In this study, a set of co-dominant markers to identify the target locus was developed by using two pairs of cross-primer PCR (PCR with confronting two-pair primer PCR-CTPP to identify the allele type of Pita. The results showed that the marker was specific, stable, accurate, time-consuming and low cost. It can be widely used in molecular marker-assisted selection breeding or in the identification and screening of Pita genotypes in rice resources.
【作者單位】： 湖北省農(nóng)業(yè)科學(xué)院糧食作物研究所糧食作物種質(zhì)創(chuàng)新與遺傳改良湖北省重點(diǎn)實(shí)驗(yàn)室;
【基金】：“七大農(nóng)作物育種”試點(diǎn)專項(xiàng)(2016YFD0102103) 科技部國際合作專項(xiàng)(2011DFB31620) 國家973計(jì)劃課題(2013CBA01405) 湖北省農(nóng)業(yè)科學(xué)院青年科學(xué)基金項(xiàng)目(2015NKYJJ02)共同資助
【分類號(hào)】：S435.111.41

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1 李時(shí)飛;四川水稻品種中抗稻瘟病基因Pita多樣性[D];四川農(nóng)業(yè)大學(xué);2012年

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