發(fā)布時間：2018-08-16 08:06

【摘要】：為了解新疆某規(guī)模化養(yǎng)殖場豬源沙門氏菌對臨床上常用抗菌藥物的耐藥情況,以及β-內(nèi)酰胺酶、16S rRNA甲基化酶和質(zhì)粒介導(dǎo)的喹諾酮耐藥(plasmid mediated quinolone resistance,PMQR)基因的流行情況,本試驗通過瓊脂稀釋法對分離的菌株進行最小抑菌濃度測定,PCR方法進行β-內(nèi)酰胺酶blaTEM、blaCMY-2、blaCTX-M、blaLAP-1、blaKPC、blaOXA和blaSHV基因,16SrRNA甲基化酶基因armA和rmtB及PMQR類基因qnrA、qnrB、qnrC、qnrD、qnrS、qepA、oqxA、oqxB和aac(6′)-Ib-cr的檢測,確定陽性菌株,分析其攜帶的基因型與耐藥表型之間的關(guān)系。分離的豬源沙門氏菌對環(huán)丙沙星和安普霉素耐藥率最高,均為77.9%(102/131),耐藥菌主要以8耐為主(29.0%,38/131)。從被檢基因中檢測出11種耐藥基因,不同基因共存有24種類型,主要以blaTEM+blaOXA+qnrS+aac(6′)-Ib-cr+oqxA+oqxB(27.6%,32/116);blaTEM+blaOXA+qnrS+oqxA+oqxB(24.1%,28/116);blaTEM+qnrS(18.0%,21/116)形式共存。該養(yǎng)殖場分離的沙門氏菌耐藥現(xiàn)象嚴重,分離耐藥菌株存在β-內(nèi)酰胺酶、16SrRNA甲基化酶和PMQR類基因共存現(xiàn)象,提示應(yīng)加強對β-內(nèi)酰胺酶、16SrRNA甲基化酶和PMQR類因子的監(jiān)控。
[Abstract]:To investigate the resistance of porcine salmonella to antibiotics commonly used in clinic, and the prevalence of 尾 -lactamase 16s rRNA methylase and plasmide-mediated (plasmid mediated quinolone resistance-resistant (plasmid mediated quinolone) gene in a large scale breeding farm in Xinjiang. In this experiment, the minimal inhibitory concentration (MEC) of the isolated strains was determined by Agar dilution method. The 尾 -lactamase blaTEMS-CMY-2 blaLACTX-mblaLAP-1 blaLAP-1 and blaSHV genes, armA and rmtB, and PMQR genes QnrAqrAqnrBnrBPQNrCnrDNNBPQNNNRPQNNNNNNNNNNNNNNNNNNNNNNNNNNNNRNNNNRNNNNNNRNNRNNNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNRNR@@ The relationship between the genotype and the phenotype of drug resistance was analyzed. The rate of resistance to ciprofloxacin and amphomycin was the highest (77.9%, 102 / 131), and 8% (29.0%) was mainly resistant to ciprofloxacin (29.0%). Eleven drug-resistant genes were detected from the tested genes. There were 24 coexisting types of different genes, mainly in the form of blaTEM blaOXA qnrS aac (6') -Ib-cr oqxA oqxB (27.632 / 116) Tem blaOXA qnrS oqxA oqxB (24.1T / 116) qnrS (18.0g / 21 / 116). The drug resistance of Salmonella isolated from the farm was serious, and the coexistence of 尾 -lactamases 16s rRNA methylases and PMQR genes in the isolates suggested that the monitoring of 尾 -lactamases 16s rRNA methylases and PMQR type factors should be strengthened.
【作者單位】： 新疆農(nóng)業(yè)大學(xué)動物醫(yī)學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金項目(31260614)
【分類號】：S852.61

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