發(fā)布時(shí)間：2018-08-16 07:28

【摘要】：APEX1是影響基因表達(dá)和氧化還原活性相關(guān)堿基修復(fù)和多功能蛋白的關(guān)鍵基因,對(duì)APEX1基因及編碼蛋白進(jìn)行生物信息學(xué)方面的深入分析,更有利于對(duì)APEX1基因相關(guān)癌癥及其他遺傳流行病學(xué)的闡述。本研究利用生物信息學(xué)分析方法以11個(gè)物種APEX1基因序列及編碼蛋白序列為研究對(duì)象,對(duì)人APEX1基因進(jìn)行了系統(tǒng)進(jìn)化分析及預(yù)測啟動(dòng)子及Cp G島,并對(duì)其蛋白質(zhì)理化性質(zhì)及結(jié)構(gòu)功能等進(jìn)行了預(yù)測分析。核酸分析結(jié)果表明,人APEX1基因包含5個(gè)外顯子,預(yù)測核心啟動(dòng)子區(qū)域?yàn)?58~208 bp。進(jìn)化分析結(jié)果顯示,人與黑猩猩的遺傳距離為0.003,親緣關(guān)系最近。蛋白預(yù)測軟件結(jié)果顯示,人APEX1蛋白主要由自由卷曲及α-螺旋結(jié)構(gòu)組成,無合適信號(hào)肽及明顯跨膜區(qū),表明該基因不參與跨膜物質(zhì)運(yùn)輸及信號(hào)轉(zhuǎn)導(dǎo)。
[Abstract]:APEX1 is a key gene affecting gene expression, redox activity and multifunctional proteins. The bioinformatics of APEX1 gene and its encoded proteins have been studied. It is more helpful for the exposition of APEX1 gene related cancer and other genetic epidemiology. In this study, APEX1 gene sequences and encoded protein sequences of 11 species were studied by bioinformatics method. The phylogenetic analysis and prediction of promoter and CP G island of human APEX1 gene were carried out. The physicochemical properties, structure and function of protein were predicted and analyzed. Nucleic acid analysis showed that the human APEX1 gene contained five exons, and the predicted core promoter region was 158208bp. Evolutionary analysis showed that the genetic distance between human and chimpanzee was 0.003. The results of protein prediction software showed that human APEX1 protein was mainly composed of free curl and 偽 -helix structure, without suitable signal peptide and obvious transmembrane region, indicating that the gene did not participate in transmembrane material transport and signal transduction.
【作者單位】： 廣東醫(yī)學(xué)院附屬醫(yī)院心血管內(nèi)科;廣東醫(yī)學(xué)院附屬醫(yī)院婦產(chǎn)科;廣東醫(yī)學(xué)院附屬醫(yī)院生殖醫(yī)學(xué)中心;
【基金】：國家自然科學(xué)基金項(xiàng)目(81300484) 廣東省自然科學(xué)基金項(xiàng)目(No.2015A030310359;2016A030313678;201-6A030310359) 廣東省醫(yī)學(xué)科學(xué)技術(shù)研究基金(No.A2016143;2016A030310359) 湛江市財(cái)政資金專項(xiàng)競爭性項(xiàng)目(2013A1006;2014A01033);湛江市財(cái)政資金專項(xiàng)備用金項(xiàng)目(2015C01006) 廣東醫(yī)學(xué)院博士啟動(dòng)基金項(xiàng)目(B2012027) 廣東醫(yī)學(xué)院附屬醫(yī)院博士啟動(dòng)基金項(xiàng)目(BJ201511)共同資助
【分類號(hào)】：Q811.4
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本文編號(hào)：2185259