【摘要】：目的:研究432株分離于陜西省楊凌及周邊城市零售雞肉中沙門氏菌的血清型分布、藥敏性及其與(氟)喹諾酮類抗菌藥物抗性相關(guān)耐藥基因的流行狀況。方法:按照玻片凝集法鑒定血清型,使用臨床和實驗室標準協(xié)會推薦的瓊脂稀釋法測定沙門氏菌的藥敏性,聚合酶鏈式反應(yīng)(polymerase chain reaction,PCR)方法確定沙門氏菌中與(氟)喹諾酮類抗生素耐藥相關(guān)基因。結(jié)果:432株沙門氏菌中除36株血清型未定外,其余396株沙門氏菌共涵蓋37個血清型,其中鼠傷寒沙門氏菌(Salmonella typhimurium,檢出率17.93%)、湯普森沙門氏菌(Salmonella thompsons,檢出率12.88%)、埃森沙門氏菌(Salmonella essen,檢出率8.84%)和嬰兒沙門氏菌(Salmonellainfants,檢出率6.57%)等血清型比較常見。沙門氏菌對磺胺異VA唑(耐藥率89.81%)耐藥最為普遍,對磺胺甲VA唑、萘啶酮酸、四環(huán)素、氨芐西林、氯霉素和阿莫西林-克拉維酸等抗生素的耐藥率均在50%以上。aac(6’)-Ib-cr基因檢出率(16.67%)最高,qnr B、qnr S和qnr A的檢出率分別為10.19%、6.71%和1.62%,且該4種基因在不同血清型菌株中分布不同。菌株攜帶qnr和aac(6’)-Ib-cr基因越多,對(氟)喹諾酮類和氨基糖苷類協(xié)同耐藥性越強。結(jié)論:陜西省楊凌及周邊城市零售雞肉源沙門氏菌的血清型種類繁多,耐藥比較普遍。
[Abstract]:Objective: to study the distribution of Salmonella serotype, drug sensitivity and the prevalence of resistance genes associated with fluoroquinolones in 432 strains of Salmonella isolated from Yang Ling in Shaanxi Province and its adjacent cities. Methods: the serum type was identified by slide agglutination, and the drug sensitivity of Salmonella was determined by Agar dilution method recommended by the Association of Clinical and Laboratory Standards. Polymerase chain reaction (PCR) was used to identify the genes related to the resistance of (fluoroquinolones) antibiotics in Salmonella by polymerase chain reaction (PCR). Results except 36 strains of Salmonella, 396 strains of Salmonella contained 37 serotypes. The serotypes of Salmonella typhimurium (17.93%), (Salmonella thompsons, (12.88%), (Salmonella essen, (8.84%) and Salmonella infantile (6.57%) were more common. Salmonella was most commonly resistant to sulfamethoxazole (89.81%), and was resistant to sulfamethoxazole, naphthalene ketoacid, tetracycline, ampicillin, ampicillin and sulfamethoxazole. The resistance rates of chloramphenicol and amoxicillin clavulanic acid were over 50%. AAC (6') -Ib-cr gene detection rate (16.67%) was the highest. The detection rates of QNR S and qnr A were 10.197.71% and 1.62B, respectively, and the four genes were distributed differently in different serotype strains. The more qnr and aac (6') -Ib-cr genes were carried, the stronger the co-resistance to fluoroquinolones and aminoglycosides. Conclusion: there are many serotypes of salmonella from chicken in Yang Ling and surrounding cities in Shaanxi province, and drug resistance is common.
【作者單位】： 西北農(nóng)林科技大學(xué)食品科學(xué)與工程學(xué)院;浙江省農(nóng)業(yè)科學(xué)院農(nóng)產(chǎn)品質(zhì)量標準研究所;浙江省農(nóng)業(yè)科學(xué)院浙江省植物有害生物防控重點實驗室;
【基金】：浙江省農(nóng)業(yè)科學(xué)院“農(nóng)產(chǎn)品質(zhì)量安全”中美國際合作項目 國家自然科學(xué)基金面上項目(31171682)
【分類號】：TS201.3;TS251.55

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