【摘要】：目的了解六盤水市彝族、漢族育齡女性葉酸代謝水平,為指導(dǎo)當(dāng)?shù)卦衅谌~酸補(bǔ)充提供一定的依據(jù)。方法選取2016—2017年期間進(jìn)行孕期體檢的育齡女性1 054例(漢族950例,彝族104例)為研究對象,采集口腔黏膜上皮脫落細(xì)胞提取基因組DNA,利用熒光定量PCR方法檢測受檢者5,10-亞甲基四氫葉酸還原酶(MTHFR)(C677T、A1298C)和甲硫氨酸合成酶還原酶(MTRR)(A66G)位點(diǎn)基因型與等位基因頻率,并通過與西南地區(qū)已報(bào)道的其他少數(shù)民族進(jìn)行比較,獲得本地區(qū)彝族、漢族上述基因位點(diǎn)多態(tài)性分布特征。結(jié)果六盤水市彝族女性MTHFR C677T位點(diǎn)基因型和等位基因頻率與當(dāng)?shù)貪h族、思南苗族、思南土家族、大理白族比較均具有統(tǒng)計(jì)學(xué)差異,其中MTHFR 677CT,677TT基因型頻率均低于其他民族。結(jié)論六盤水市彝族與漢族育齡女性葉酸代謝關(guān)鍵酶基因位點(diǎn)多態(tài)性特征有所區(qū)別,可以有針對性地制定符合當(dāng)?shù)孛褡逄卣鞯膫�(gè)性化葉酸補(bǔ)充方案。
[Abstract]:Objective to investigate the folic acid metabolism of Yi and Han women in Liupanshui city, and to provide evidence for guiding folic acid supplementation during pregnancy. Methods 1 054 women of childbearing age (950 Han and 104 Yi) who underwent physical examination during pregnancy during 2016-2017 were selected as subjects. Genomic DNA was extracted from exfoliated cells from oral mucosal epithelium. The genotypes and allelic frequencies of 510-methylene tetrahydrofolate reductase (MTHFR) (C677TnA1298C) and methionine synthase reductase (MTRR) (A66G (MTRR) (A66G) were detected by fluorescence quantitative PCR. By comparing with other ethnic minorities reported in Southwest China, the distribution characteristics of the above gene loci were obtained in Yi nationality and Han nationality. Results the genotype and allele frequency of MTHFR C677T locus in Yi women in Liupanshui city were significantly different from those of Han nationality, Sinan Miao nationality, Sinan Tujia nationality and Dali Bai nationality, and the frequency of MTHFR 677 CTN 677TT genotype was lower than that of other nationalities. Conclusion the polymorphism characteristics of folic acid metabolism key enzyme gene loci in Yi nationality and Han nationality in Liupanshui city are different, and the individualized folic acid supplementation scheme can be formulated in accordance with the local national characteristics.
【作者單位】： 六盤水市婦幼保健院;上海靶向分子醫(yī)學(xué)研究所;中國疾病預(yù)防控制中心婦幼保健中心;
【基金】：中國疾病預(yù)防控制中心婦幼保健中心婦幼保健分子遺傳醫(yī)學(xué)研究專項(xiàng)計(jì)劃(FY-ZX-ZD-0313)~~
【分類號】：R711

【相似文獻(xiàn)】

相關(guān)期刊論文 前4條

1 周艷紅;王新;;葉酸代謝與出生缺陷的關(guān)系研究[J];醫(yī)學(xué)臨床研究;2006年09期

2 修霞;郎翠紅;牟瑩瑩;;葉酸代謝障礙對孕婦及胎兒的不良影響[J];中國優(yōu)生與遺傳雜志;2013年01期

3 程寧,白亞娜;葉酸代謝與神經(jīng)管畸形的關(guān)聯(lián)研究進(jìn)展[J];蘭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年02期

4 廖亞平;鮑明升;梁玉華;;葉酸代謝相關(guān)基因多態(tài)性與Down綜合征發(fā)生的關(guān)系[J];蚌埠醫(yī)學(xué)院學(xué)報(bào);2009年10期

相關(guān)會(huì)議論文 前1條

1 李海波;王挺;王本敬;朱芹;李瓊;李紅;陳瑛;;孕婦葉酸代謝相關(guān)基因多態(tài)性與孕中期血清學(xué)水平異常的相關(guān)性[A];第十二次全國醫(yī)學(xué)遺傳學(xué)學(xué)術(shù)會(huì)議論文匯編[C];2014年

相關(guān)碩士學(xué)位論文 前2條

1 朱芹;葉酸代謝通路關(guān)鍵基因單核苷酸多態(tài)與早產(chǎn)的相關(guān)性研究[D];南京醫(yī)科大學(xué);2014年

2 梁爽;葉酸代謝通路相關(guān)基因SNPs與孕婦同型半胱氨酸代謝能力的相關(guān)性分析[D];復(fù)旦大學(xué);2012年

，

本文編號：2165043