【摘要】：目的了解六盤(pán)水市彝族、漢族育齡女性葉酸代謝水平,為指導(dǎo)當(dāng)?shù)卦衅谌~酸補(bǔ)充提供一定的依據(jù)。方法選取2016—2017年期間進(jìn)行孕期體檢的育齡女性1 054例(漢族950例,彝族104例)為研究對(duì)象,采集口腔黏膜上皮脫落細(xì)胞提取基因組DNA,利用熒光定量PCR方法檢測(cè)受檢者5,10-亞甲基四氫葉酸還原酶(MTHFR)(C677T、A1298C)和甲硫氨酸合成酶還原酶(MTRR)(A66G)位點(diǎn)基因型與等位基因頻率,并通過(guò)與西南地區(qū)已報(bào)道的其他少數(shù)民族進(jìn)行比較,獲得本地區(qū)彝族、漢族上述基因位點(diǎn)多態(tài)性分布特征。結(jié)果六盤(pán)水市彝族女性MTHFR C677T位點(diǎn)基因型和等位基因頻率與當(dāng)?shù)貪h族、思南苗族、思南土家族、大理白族比較均具有統(tǒng)計(jì)學(xué)差異,其中MTHFR 677CT,677TT基因型頻率均低于其他民族。結(jié)論六盤(pán)水市彝族與漢族育齡女性葉酸代謝關(guān)鍵酶基因位點(diǎn)多態(tài)性特征有所區(qū)別,可以有針對(duì)性地制定符合當(dāng)?shù)孛褡逄卣鞯膫�(gè)性化葉酸補(bǔ)充方案。
[Abstract]:Objective to investigate the folic acid metabolism of Yi and Han women in Liupanshui city, and to provide evidence for guiding folic acid supplementation during pregnancy. Methods 1 054 women of childbearing age (950 Han and 104 Yi) who underwent physical examination during pregnancy during 2016-2017 were selected as subjects. Genomic DNA was extracted from exfoliated cells from oral mucosal epithelium. The genotypes and allelic frequencies of 510-methylene tetrahydrofolate reductase (MTHFR) (C677TnA1298C) and methionine synthase reductase (MTRR) (A66G (MTRR) (A66G) were detected by fluorescence quantitative PCR. By comparing with other ethnic minorities reported in Southwest China, the distribution characteristics of the above gene loci were obtained in Yi nationality and Han nationality. Results the genotype and allele frequency of MTHFR C677T locus in Yi women in Liupanshui city were significantly different from those of Han nationality, Sinan Miao nationality, Sinan Tujia nationality and Dali Bai nationality, and the frequency of MTHFR 677 CTN 677TT genotype was lower than that of other nationalities. Conclusion the polymorphism characteristics of folic acid metabolism key enzyme gene loci in Yi nationality and Han nationality in Liupanshui city are different, and the individualized folic acid supplementation scheme can be formulated in accordance with the local national characteristics.
【作者單位】： 六盤(pán)水市婦幼保健院;上海靶向分子醫(yī)學(xué)研究所;中國(guó)疾病預(yù)防控制中心婦幼保健中心;
【基金】：中國(guó)疾病預(yù)防控制中心婦幼保健中心婦幼保健分子遺傳醫(yī)學(xué)研究專(zhuān)項(xiàng)計(jì)劃(FY-ZX-ZD-0313)~~
【分類(lèi)號(hào)】：R711

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