【摘要】：目的觀察過表達(dá)S100A13基因的甲狀腺癌TT細(xì)胞侵襲能力變化情況。方法分別用pc DNA3.1/NT-GFP-S100A13表達(dá)質(zhì)粒和pc DNA3.1/NT-GFP空質(zhì)粒轉(zhuǎn)染TT細(xì)胞,構(gòu)建空載體p CDNA3.1/NT-GFP-TT細(xì)胞系(空載體組)和S100A13穩(wěn)定過表達(dá)甲狀腺癌TT細(xì)胞系(S100A13過表達(dá)組),采用Transwell侵襲實(shí)驗(yàn)觀察兩組細(xì)胞的侵襲能力,蛋白印跡法檢測(cè)兩組細(xì)胞酸性成纖維細(xì)胞生長(zhǎng)因子1(FGF-1)、細(xì)胞周期蛋白E(Cyclin E)、CD31蛋白表達(dá)。結(jié)果 S100A13過表達(dá)組、空載體組穿出基質(zhì)膜的細(xì)胞數(shù)分別為(83.7±5.0)與(40.0±1.7)個(gè),兩組比較P0.01。S100A13過表達(dá)組、空載體組細(xì)胞FGF-1蛋白相對(duì)表達(dá)量分別為1.224±0.124、0.837±0.152,Cyclin E蛋白相對(duì)表達(dá)量分別為1.034±0.131、0.459±0.032,CD31蛋白相對(duì)表達(dá)量分別為1.147±0.144、0.312±0.071,兩組比較,P0.05或0.01。結(jié)論過表達(dá)S100A13基因的甲狀腺癌TT細(xì)胞侵襲能力增強(qiáng),這可能與S100A13促進(jìn)FGF-1、Cyclin E、CD31蛋白表達(dá)有關(guān)。
[Abstract]:Objective to observe the change of invasiveness of TT cells in thyroid carcinoma which expressed S100A13 gene. Methods TT cells were transfected with PC DNA3.1/NT-GFP-S100A13 expression plasmid and PC DNA3.1/NT-GFP empty plasmid, respectively. The empty vector p CDNA3.1/NT-GFP-TT cell line (empty vector group) and the TT cell line with stable overexpression of S100A13 (S100A13 overexpression group) were constructed. The invasiveness of the two groups was observed by Transwell invasion assay. The expression of acidic fibroblast growth factor 1 (FGF-1) and cyclin E (Cyclin E) CD31 protein were detected by Western blot. Results the number of cells piercing the matrix membrane in S100A13 overexpression group and empty vector group were (83.7 鹵5.0) and (40.0 鹵1.7), respectively. The relative expression of FGF-1 protein in empty vector group was 1.224 鹵0.124 鹵0.837 鹵0.152 and 1.034 鹵0.131 鹵0.459 鹵0.032, respectively. The relative expression of FGF-1 protein was 1.147 鹵0.144 鹵0.312 鹵0.071, respectively. Conclusion the invasiveness of TT cells in thyroid carcinoma with overexpression of S100A13 gene is enhanced, which may be related to the enhanced expression of FGF-1 Cyclin EtoCD31 protein by S100A13.
【作者單位】： 郴州市第一人民醫(yī)院;
【基金】：湖南省醫(yī)藥衛(wèi)生科研計(jì)劃課題項(xiàng)目(B2014-176)
【分類號(hào)】：R736.1

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