【摘要】：板栗疫病菌(Cryphonectria parasitica)是引起板栗疫病的一種絲狀子囊菌。UV57是一個不支持病毒復(fù)制且致病力喪失的C.parasitica紫外誘變突變株。前期蛋白質(zhì)組研究結(jié)果顯示,蛋白86233(一種甲基轉(zhuǎn)移酶)只在UV57中出現(xiàn),而在野生型對照株EP155中沒有檢測到。為研究編碼86233蛋白的cpomt基因的功能,本研究通過同源重組方法成功構(gòu)建了缺失突變體Δcpomt及其互補(bǔ)轉(zhuǎn)化株。與野生型EP155相比,Δcpomt菌株生長緩慢,色素分泌減少,產(chǎn)孢量降低,菌絲形態(tài)異常,對休眠板栗樹枝的致病性顯著降低。而在互補(bǔ)轉(zhuǎn)化株Δcpomt-com中,這些表型及致病力變化均可以恢復(fù)到野生型水平。cpomt基因的缺失對低毒病毒CHV1-EP713的復(fù)制累積量沒有影響,但導(dǎo)致抗逆相關(guān)基因G-α,產(chǎn)孢基因CLS-32,色素合成酶基因PKS轉(zhuǎn)錄水平明顯下調(diào)。本研究為闡明甲基轉(zhuǎn)移酶在病原真菌中的作用提供了新的知識。
[Abstract]:Cryphonectria parasitica is a filamentous ascomycetes causing chestnut blight. UV57 is a C.parasitica UV mutant that does not support virus replication and loses pathogenicity. Previous proteome studies showed that protein 86233 (a methyltransferase) was only found in UV57, but not in wild type control strain EP155. In order to study the function of cpomt gene encoding 86233 protein, the deletion mutant 螖 cpomt and its complementary transformation strain were successfully constructed by homologous recombination method. Compared with wild type EP155, 螖 cpomt strain grew slowly, pigment secretion decreased, sporulation decreased, hyphal morphology was abnormal, pathogenicity of dormant chestnut branches was significantly decreased. In the complementary transformation strain 螖 cpomt-com, these phenotypic and pathogenicity changes could recover to the wild-type level. The deletion of cpomt gene had no effect on the replication accumulation of CHV1-EP713. However, the transcription level of G- 偽, CLS-32 and PKS was down-regulated. This study provides a new understanding of the role of methyltransferase in pathogenic fungi.
【作者單位】： 廣西大學(xué)生命科學(xué)與技術(shù)學(xué)院;廣西大學(xué)亞熱帶農(nóng)業(yè)生物資源保護(hù)與利用國家重點(diǎn)實(shí)驗室;
【基金】：國家自然科學(xué)基金(31260027) 廣西自然科學(xué)基金(2014GXNSFBA118064)共同資助
【分類號】：S436.64
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本文編號：2122783