本文選題：結(jié)核分枝桿菌 + 間隔區(qū)寡核苷酸分型��； 參考：《現(xiàn)代預(yù)防醫(yī)學(xué)》2017年24期

【摘要】：目的探究楚雄彝族自治州流行的結(jié)核分枝桿菌基因分型情況與耐藥的相關(guān)性。方法收集2014年4月-2015年4月在楚雄分離得到結(jié)核分枝桿菌菌株,采用比例法檢測(cè)6種抗結(jié)核藥物的敏感性,采用間隔區(qū)寡核苷酸分型法(Spoligotyping)進(jìn)行基因分型。結(jié)果本研究共分離獲得103株結(jié)核分枝桿菌臨床分離株,其總體耐藥率和耐多藥率分別為15.33%、6.80%。Spoligotyping分型可分為北京基因家族(Beijing family)75株(72.82%)及非北京基因家族(NonBeijing family)28株(27.18%)2個(gè)大基因群,共包括18個(gè)基因型,含6個(gè)基因簇及12個(gè)獨(dú)立基因型,4個(gè)獨(dú)立基因型為本研究新發(fā)現(xiàn)基因型。北京基因家族中,典型北京基因型70株(93.33%,70/75)為主要的流行株;非北京基因家族中,T1家族13株(46.43%,13/28)為主要的流行株。北京基因家族與非北京基因家族總體耐藥率分別為17.33%(13/75)、10.71%(3/28),二者差異無(wú)統(tǒng)計(jì)學(xué)意義(χ~2=0.270,P=0.603);耐多藥率分別為8%(6/75)、3.57%(1/28),二者差異無(wú)統(tǒng)計(jì)學(xué)意義(χ~2=0.126,P=0.723)。結(jié)論北京基因家族為楚雄彝族自治州流行的優(yōu)勢(shì)菌株,與非北京基因家族的耐藥性無(wú)統(tǒng)計(jì)學(xué)差異。
[Abstract]:Objective to investigate the relationship between the genotyping of Mycobacterium tuberculosis and drug resistance in Chuxiong Yi Autonomous Prefecture. Methods Mycobacterium tuberculosis strains were isolated from Chuxiong from April 2014 to April 2015. The susceptibility of 6 antituberculous drugs was detected by proportional method and genotyping by Spoligotyping. Results A total of 103 clinical isolates of Mycobacterium tuberculosis were isolated. The total drug resistance rate and multidrug resistance rate were 15.33 and 6.80 Spoligotyping, respectively. There were 75 strains (72.82%) of Beijing family and 28 strains (27.18%) of nonBeijing family. There were 18 genotypes, including 6 gene clusters and 12 independent genotypes, and 4 independent genotypes were the new genotypes found in this study. In the Beijing gene family, 70 typical Beijing genotypes (93.33 / 70 / 75) were the main epidemic strains, and 13 (46.43 / 28) were the main epidemic strains in the non-Beijing gene families. The overall drug resistance rates of Beijing gene family and non-Beijing gene family were 17.33% (13 / 75) and 10.71% (3 / 28), respectively. There was no significant difference between them (蠂 ~ (2 / 2) 0.270) and multidrug resistance (8% (6 / 75) or 3.57% (1 / 28), respectively). Conclusion the Beijing gene family is the dominant strain in Chuxiong Yi Autonomous Prefecture, and there is no significant difference in drug resistance between Beijing gene family and non-Beijing gene family.
【作者單位】： 云南省疾病預(yù)防控制中心結(jié)核病防治所;
【分類號(hào)】：R52

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