本文選題：亞麻 + 韌皮纖維��； 參考：《中國(guó)農(nóng)業(yè)科學(xué)》2017年13期

【摘要】：【目的】亞麻在快速生長(zhǎng)期其韌皮纖維細(xì)胞發(fā)育在SP(the snap point)點(diǎn)上下端分別經(jīng)歷細(xì)胞伸長(zhǎng)和次生細(xì)胞壁加厚2個(gè)不重疊時(shí)期。研究亞麻快速生長(zhǎng)期不同組織、不同時(shí)期、不同器官中與細(xì)胞壁形成相關(guān)的β-半乳糖苷酶(Lu BGALs)、纖維素合酶(Lu CESAs)等家族基因的表達(dá)譜,探討快速生長(zhǎng)期亞麻韌皮纖維細(xì)胞細(xì)胞壁的發(fā)育模式,為改善亞麻纖維產(chǎn)量提供理論依據(jù)。【方法】以生長(zhǎng)45 d的亞麻根、莖韌皮纖維、葉為材料,用透射電鏡觀察并測(cè)量莖韌皮纖維細(xì)胞細(xì)胞壁結(jié)構(gòu)和厚度,采用實(shí)時(shí)熒光定量(q RT-PCR)方法,研究亞麻快速生長(zhǎng)期Lu BGALs和Lu CESAs等細(xì)胞壁形成相關(guān)的基因在亞麻韌皮纖維不同階段的表達(dá)特點(diǎn)。【結(jié)果】在SP點(diǎn)上部TOP端纖維細(xì)胞細(xì)胞壁薄,約110 nm;緊鄰SP點(diǎn)莖中部的MID區(qū)(約500 nm)和莖下部的BOT區(qū)(約650 nm),細(xì)胞壁厚度明顯增厚,細(xì)胞壁質(zhì)地均一,沒有明顯的分層現(xiàn)象,說明SP點(diǎn)中部和下部韌皮纖維細(xì)胞細(xì)胞壁已經(jīng)開始加厚但還未進(jìn)入次生壁加厚階段,與TOP明顯不同。亞麻L(zhǎng)u BGAL1在TOP區(qū)的表達(dá)顯著低于MID區(qū)和BOT區(qū),表明其主要參與纖維細(xì)胞細(xì)胞壁加厚過程。而Lu BGAL3、Lu BGAL6、Lu BGAL9在TOP區(qū)表達(dá)最高,MID區(qū)次之,表明此類基因主要參與亞麻韌皮細(xì)胞伸長(zhǎng)和細(xì)胞壁重建過程。Lu BGAL5在幼嫩的TOP區(qū)表達(dá)量高,在亞麻莖較為成熟的MID區(qū)較低,說明Lu BGAL5在細(xì)胞壁形成過程中起作用。其他BGALs基因的表達(dá)量均較低。在亞麻莖幼嫩的TOP區(qū)纖維細(xì)胞中,亞麻纖維素合酶基因Lu CESA1、Lu CESA3、Lu CESA7、Lu CESA8、Lu CESA9和Lu CESA10都檢測(cè)出較高的表達(dá)量,且明顯高于其在MID區(qū)和BOT區(qū)的表達(dá)。其中Lu CESA3和Lu CESA10在MID區(qū)的表達(dá)顯著低于BOT區(qū),其他幾個(gè)CESAs基因在MID和BOT的表達(dá)并無明顯差異。結(jié)合這些基因在亞麻快速生長(zhǎng)期不同器官中的表達(dá)模式,結(jié)果說明,亞麻中6個(gè)CESA(Lu CESA1、Lu CESA3、Lu CESA7、Lu CESA8、Lu CESA9和Lu CESA10)主要促進(jìn)亞麻韌皮纖維細(xì)胞的伸長(zhǎng)。Lu Su Sy在幼莖韌皮纖維細(xì)胞中表達(dá)量高,表明亞麻莖伸長(zhǎng)和加粗需要大量能量。Lu XTH4在亞麻細(xì)胞壁發(fā)育過程中發(fā)揮作用�！窘Y(jié)論】快速生長(zhǎng)期亞麻莖韌皮纖維細(xì)胞細(xì)胞壁沒有次生加厚過程;Lu BGAL3、Lu BGAL5、Lu BGAL6、Lu BGAL9、Lu CESA1、Lu CESA3、Lu CESA9和Lu CESA10在亞麻細(xì)胞壁細(xì)胞伸長(zhǎng)過程中起作用;Lu BGAL1主要促進(jìn)亞麻細(xì)胞壁加厚過程;Lu Su Sy和Lu XTH4在亞麻細(xì)胞壁發(fā)育中發(fā)揮作用。
[Abstract]:[objective] the development of phloem fibroblasts at SP (the snap point) site in fast growing stage of flax underwent two stages: cell elongation and secondary cell wall thickening. To study the expression profiles of 尾 -galactosidase (Lu BGALs), cellulose synthase (Lu CESAs) and other family genes related to cell wall formation in different tissues, different stages and different organs of flax. To study the development pattern of cell wall of flax phloem fiber in rapid growing period, and to provide theoretical basis for improving the yield of flax fiber. [methods] Flax root, stem phloem fiber and leaf were used as materials for 45 days of growth. The cell wall structure and thickness of phloem fiber were observed and measured by transmission electron microscope (TEM). To study the expression characteristics of cell wall formation related genes such as Lu BGALs and Lu CESAs in the fast growing stage of flax at different stages of flax phloem fiber. About 110nm, close to the mid region of the stem of SP point (about 500nm) and the bot region of the lower part of the stem (about 650Nm), the thickness of the cell wall is obviously thickened, the texture of the cell wall is uniform, and there is no obvious stratification. The results showed that the cell wall of the middle and lower phloem fibers in SP point was thickened but not in the stage of secondary wall thickening, which was different from top. The expression of Lu BGAL1 in top region was significantly lower than that in mid and bot regions, indicating that Lu BGAL1 was mainly involved in fiber wall thickening. Lu BAL3, Lu BGAL6, Lu BGAL9 expressed the highest level of mid in top region, indicating that these genes were mainly involved in the process of cell elongation and cell wall reconstruction of flax phloem. Lu BGAL5 was highly expressed in the young top region, and lower in mid region, where the flax stem was more mature. These results suggest that Lu BGAL5 plays an important role in cell wall formation. The expression of other BGALs genes was low. In the top region fibroblasts with young flax stems, the high expression levels of the linseed cellulosic synthase gene Lu CESA1, Lu CESA3, Lu CESA7, Lu CESA8, Lu CESA9 and Lu CESA10 were detected, which were significantly higher than those in mid and bot regions. The expression of Lu CESA3 and Lu CESA10 in mid region was significantly lower than that in bot region. The expression patterns of these genes in different organs of flax at rapid growth stage were combined. The results showed that six CESA (Lu CESA1, Lu CESA3, Lu CESA7, Lu CESA8, Lu CESA9 and Lu CESA10) mainly promoted the high expression of Lu Su Sy in flax phloem fiber cells. [conclusion] there is no secondary thickening process in the cell wall of flax stem phloem fiber. [conclusion] there is no secondary thickening process in the cell wall of flax stem phloem fiber. [conclusion] there is no secondary thickening process in the cell wall of flax stem phloem fiber. [conclusion] Lu BGAL3, Lu BGAL5, Lu BGAL6, Lu BGAL9 Lu CESA1, CESA3 Lu, CESA3 Lu, CESA1, CESA3 Lu. CESA9 and Lu CESA10 play an important role in the cell wall cell elongation of flax. Lu BGAL1 mainly promotes the cell wall thickening process of flax. Lu Su Sy and Lu XTH4 play roles in the development of flax cell wall.
【作者單位】： 新疆大學(xué)生命科學(xué)與技術(shù)學(xué)院;
【基金】：國(guó)家自然科學(xué)基金(31160056/C020408)
【分類號(hào)】：S563.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 汪開治;亞麻用途多[J];植物雜志;2002年06期

2 王維亮;;纖維亞麻的引進(jìn)及栽培技術(shù)[J];現(xiàn)代農(nóng)業(yè)科技;2006年11期

3 李建美;張玉華;朱以維;;大理州冬亞麻氣候區(qū)劃[J];云南農(nóng)業(yè)科技;2007年01期

4 姬妍茹;;根癌農(nóng)桿菌介導(dǎo)的亞麻遺傳轉(zhuǎn)化研究進(jìn)展[J];黑龍江紡織;2008年03期

5 趙德寶;郭永利;陳浩;宋憲友;楊學(xué);豐志強(qiáng);謝民生;余涌;;新疆亞麻良種繁殖技術(shù)[J];中國(guó)麻業(yè)科學(xué);2010年02期

6 李愛榮;米君;曲志華;張麗麗;喬海明;;高產(chǎn)抗病油用亞麻新品種壩亞12號(hào)選育[J];中國(guó)麻業(yè)科學(xué);2010年03期

7 鄭洪艷;;纖維用亞麻高產(chǎn)栽培技術(shù)[J];科學(xué)種養(yǎng);2010年09期

8 李秋芝;;纖維亞麻雜交育種的方法和技術(shù)[J];黑龍江科技信息;2011年14期

9 吳建忠;;亞麻全基因組DNA的提取及分析[J];黑龍江農(nóng)業(yè)科學(xué);2011年07期

10 趙曉云;賀慶安;;旱地籽用亞麻栽培試驗(yàn)示范[J];青海農(nóng)技推廣;2011年04期

相關(guān)會(huì)議論文 前1條

1 郝冬梅;邱財(cái)生;于文靜;陳信波;鄧欣;龍松華;賈婉琪;郭媛;王玉富;;亞麻RAPD標(biāo)記分子身份證體系的構(gòu)建與遺傳多樣性分析[A];2010中國(guó)作物學(xué)會(huì)學(xué)術(shù)年會(huì)論文摘要集[C];2010年

相關(guān)重要報(bào)紙文章 前2條

1 臨滄地區(qū)植保站 韋麗莉 永德縣植保站 王朝蓮;亞麻主要病蟲害防治[N];云南科技報(bào);2004年

2 祥云縣農(nóng)科所 陸世民;纖用型亞麻特征特性及栽培[N];云南科技報(bào);2003年

相關(guān)博士學(xué)位論文 前2條

1 高珍妮;油用亞麻抗倒伏特性及對(duì)栽培措施的響應(yīng)[D];甘肅農(nóng)業(yè)大學(xué);2015年

2 斯欽巴特爾;顯性雄性核不育亞麻雄性不育相關(guān)基因的研究[D];內(nèi)蒙古大學(xué);2009年

相關(guān)碩士學(xué)位論文 前10條

1 王利鵑;不同播期和密度對(duì)冬季亞麻生長(zhǎng)發(fā)育的影響[D];西南大學(xué);2015年

2 劉冰;除草劑對(duì)油用亞麻生長(zhǎng)發(fā)育的影響[D];山西農(nóng)業(yè)大學(xué);2016年

3 亢魯毅;油用亞麻種質(zhì)資源的基礎(chǔ)分類[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2009年

4 孫燕華;亞麻脫膠微生物的篩選、鑒定及其多樣性研究[D];內(nèi)蒙古大學(xué);2009年

5 楊崇慶;油用亞麻雜種優(yōu)勢(shì)利用研究[D];甘肅農(nóng)業(yè)大學(xué);2009年

6 頡瑞霞;油用亞麻（胡麻）再生體系和農(nóng)桿菌介導(dǎo)的遺傳轉(zhuǎn)化體系研究[D];甘肅農(nóng)業(yè)大學(xué);2010年

7 王麗爽;亞麻原生質(zhì)體制備、融合及培養(yǎng)技術(shù)[D];大連工業(yè)大學(xué);2010年

8 林琳;油用亞麻雜交組合的性狀和過氧化物酶同工酶及ISSR分子標(biāo)記分析[D];內(nèi)蒙古農(nóng)業(yè)大學(xué);2010年

9 葛春輝;亞麻胚性愈傷的誘導(dǎo)及耐鹽愈傷的生理生化特性[D];新疆大學(xué);2007年

10 苗書魁;‘范妮’亞麻離體形態(tài)發(fā)生及‘雙亞五號(hào)’亞麻與羅布麻原生質(zhì)體融合[D];新疆大學(xué);2010年

，

本文編號(hào)：2092938