本文選題：苦蕎 + 查爾酮合酶基因�。� 參考：《植物科學(xué)學(xué)報(bào)》2017年04期

【摘要】：采用染色體步移技術(shù),從苦蕎(Fagopyrum tataricum Gaertn.)中克隆獲得Ft CHS1基因5'端側(cè)翼序列,共1038 bp,將其命名為PFt CHS1。生物信息學(xué)分析表明,PFt CHS1中A/T堿基含量為63.5%,含有4個(gè)可能的轉(zhuǎn)錄起始位點(diǎn),分別位于起始密碼子上游-684~-734、-692~-742、-920~-970、-929~-979 bp處,該序列包含TATA-Box和CAAT-Box等啟動(dòng)子核心元件以及與光、低溫和激素應(yīng)答等相關(guān)的功能元件。本研究進(jìn)一步構(gòu)建了PFt CHS1-p BI101植物表達(dá)載體,并瞬時(shí)轉(zhuǎn)化擬南芥(Arabidopsis thaliana L.)葉片,結(jié)果顯示該序列可驅(qū)動(dòng)GUS報(bào)告基因的表達(dá)。低溫(4℃)和光照(UV-B)處理苦蕎幼芽后,采用熒光定量PCR技術(shù)分析Ft CHS1基因的表達(dá)量,結(jié)果表明PFt CHS1可響應(yīng)低溫和紫外環(huán)境脅迫,從而引起Ft CHS1基因表達(dá)量發(fā)生變化。
[Abstract]:The chromosome step technique was used to study the genetic properties of Tartary buckwheat (Fagopyrum tataricum Gaertn.) The 5 'flanking sequence of Ft CHS1 gene was obtained, which was named PFT CHS1. Bioinformatics analysis showed that the content of the A / T base in PFT CHS1 was 63.55.It contained four possible transcriptional initiation sites, located at the upper reaches of the initiation codon -684N -734U -692U -742 + -970-920U -970-929N -979 BP. The sequence contained promoter core elements such as TATA-Box and CAAT-Box, as well as light, and light, respectively. Hypothermia and hormone response and other related functional elements. In this study, the plant expression vector PFT CHS1-p BI101 was constructed and transformed into Arabidopsis thaliana (Arabidopsis thaliana L. The results showed that this sequence could drive the expression of Gus reporter gene. The expression of Ft CHS1 gene was analyzed by fluorescence quantitative PCR after treatment with low temperature (4 鈩，

本文編號(hào)：2086089