本文選題：沙雷氏菌 + 脂肪酶��； 參考：《福州大學(xué)學(xué)報(bào)(自然科學(xué)版)》2016年05期

【摘要】：克隆一個(gè)源于北極凍土沙雷氏菌的脂肪酶基因lip18,實(shí)現(xiàn)其在大腸桿菌中的表達(dá),并進(jìn)行酶學(xué)性質(zhì)研究.克隆脂肪酶基因lip18,并構(gòu)建p ET-28a(+)-lip18重組表達(dá)載體,導(dǎo)入大腸桿菌BL21(DE3)中,誘導(dǎo)優(yōu)化重組蛋白表達(dá),并研究其酶學(xué)性質(zhì).克隆得到脂肪酶基因lip18,全長為1 842 bp,編碼614個(gè)氨基酸.重組菌的誘導(dǎo)溫度對蛋白表達(dá)影響很大,在最適誘導(dǎo)溫度為20℃時(shí)脂肪酶大量表達(dá),重組脂肪酶的相對分子質(zhì)量約為65 ku.酶學(xué)性質(zhì)研究表明:重組酶高效水解C10~C16的中、長鏈脂肪酸,最適作用溫度30℃,最適作用pH值7.0,并且在0℃條件下有一定的催化活性,熱穩(wěn)定性差,是低溫中性脂肪酶;同時(shí),對有機(jī)溶劑有較好的耐受性.
[Abstract]:Lipase gene lip18 was cloned from Shareh's bacteria in the Arctic, and its expression in Escherichia coli was achieved and its enzymatic properties were studied. Lipase gene lip18 was cloned and pET-28a () -lip18 recombinant expression vector was constructed. The recombinant protein was transformed into E. coli BL21 (DE3), and its enzymatic properties were studied. Lip18 gene was cloned and its length was 1 842 BP, encoding 614 amino acids. The induction temperature of recombinant bacteria had a great influence on the protein expression, and the relative molecular weight of the recombinant lipase was about 65 ku. at the optimal induction temperature of 20 鈩，

本文編號(hào)：2073392