本文選題：西伯利亞鱘 + Sox　； 參考：《海洋漁業(yè)》2017年06期

【摘要】：采用RACE技術(shù)獲得西伯利亞鱘(Acipenser baerii)Sox17基因cDNA全長,序列分析表明,西伯利亞鱘Sox17基因cDNA全長2 671 bp,包括588 bp的5'端非翻譯區(qū),901 bp的3'端非翻譯區(qū)和編碼393個氨基酸殘基的1 183 bp開放閱讀框。氨基酸序列比對顯示,該基因具較高的保守性,與塞內(nèi)加爾多鰭魚(Polypterus senegalus)的相似度最高為72%。系統(tǒng)進化分析表明,西伯利亞鱘與塞內(nèi)加爾多鰭魚聚為一支,且支持率為95%。以延伸因子efla(elongation factor 1-alpha)作為內(nèi)參基因,對Sox17基因在西伯利亞鱘各組織的相對表達量進行分析,發(fā)現(xiàn)Sox17基因在不同組織中均有表達,但表達具有明顯的組織差異性。在腦中表達量最高,而在腎、血液、眼、腸和胸鰭5個組織中表達量均較低。同時,Sox17基因在西伯利亞鱘不同發(fā)育時期均有表達,但表達具有一定的時間差異性。在寬神經(jīng)板期的表達量高,相當于表達量最低點11日齡仔魚的167倍。本研究為量化西伯利亞鱘胚胎發(fā)育分化過程中相關(guān)基因提供了參照工具,同時可為今后深入研究西伯利亞鱘Sox17在胚胎發(fā)育過程中的作用提供基礎(chǔ)參考資料。
[Abstract]:The full-length cDNA of (Acipenser baerii) Sox17 gene of Acipenser Siberian Sturgeon was obtained by race technique. The full-length cDNA of Sox17 gene of Acipenser Siberia Sturgeon is 2 671 BP, including 588 BP 5'untranslated region 901 BP untranslated region and 1 183 BP open reading frame encoding 393 amino acid residues. Amino acid sequence alignment showed that the gene was highly conserved and had the highest similarity to the Senegalese polyfin fish (Polypterus senegalus). Phylogenetic analysis showed that the Siberian Sturgeon and the Senegalese polyfin were one and the support rate was 95. The relative expression of Sox17 gene in various tissues of Acipenser sibirica was analyzed by using the extension factor efla (elongation factor 1-alpha as internal reference gene. It was found that Sox17 gene was expressed in different tissues, but the expression of Sox17 gene was different in different tissues. The highest expression was found in the brain, but low in the kidney, blood, eye, intestine and pectoral fin. At the same time, Sox17 gene was expressed in different developmental stages of Acipenser sibirica, but the expression had some time difference. The expression level in the broad nerve plate stage was 167 times of that of the 11 day old larva at the lowest level of expression. This study provides a reference tool for quantifying the genes involved in embryonic development and differentiation of Acipenser Siberian Sturgeon, and provides a basic reference for the further study of the role of Sox17 in embryonic development of Acipenser Siberian Sturgeon.
【作者單位】： 中國水產(chǎn)科學研究院東海水產(chǎn)研究所農(nóng)業(yè)部東海與遠洋漁業(yè)資源開發(fā)利用重點實驗室;上海海洋大學水產(chǎn)與生命學院;
【基金】：國家自然科學基金項目(31302161)
【分類號】：Q78;S917.4

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