本文選題：杜仲 + MVA途徑�。� 參考：《植物研究》2017年01期

【摘要】：通過(guò)對(duì)杜仲基因組分析,篩選并克隆出MVA途徑和MEP途徑的相關(guān)基因全長(zhǎng)(Eu DXR,Eu MCT,Eu CMK,Eu MDS,Eu ACOT,Eu HMGS和Eu HMGR),并通過(guò)生物信息學(xué)方法分析其結(jié)構(gòu)特征,結(jié)果表明上述基因與其他已知物種相應(yīng)基因的相似度達(dá)73%~85%。通過(guò)構(gòu)建亞細(xì)胞定位表達(dá)載體,并瞬時(shí)轉(zhuǎn)化煙草下表皮細(xì)胞后激光共聚焦顯微鏡下觀察顯示,Eu DXR,Eu MCT,Eu CMK,Eu MDS基因編碼蛋白定位于葉綠體,Eu ACOT和Eu HMGR基因編碼蛋白定位于內(nèi)質(zhì)網(wǎng),Eu HMGS基因編碼蛋白定位于細(xì)胞質(zhì)膜。利用轉(zhuǎn)錄組測(cè)序技術(shù)分析上述基因的時(shí)空表達(dá)特性表明,MEP途徑相關(guān)基因在杜仲葉片中大量表達(dá),而MVA途徑相關(guān)基因在杜仲幼果中大量表達(dá),且杜仲幼果比葉片中的橡膠含量高,因此,推斷MVA途徑在杜仲橡膠合成中占主導(dǎo)作用。
[Abstract]:Based on the genomic analysis of Eucommia ulmoides, the full length of MVA and MEP related genes (EU DXRN EU MCTN EU CMKGG EU MDSU ACOTU HMGS and EU HMGR) were screened and cloned, and their structural characteristics were analyzed by bioinformatics. The results showed that the similarity between these genes and the corresponding genes of other known species was 73%. Subcellular localization expression vector was constructed. After transient transformation of tobacco epidermis cells, laser confocal microscopy showed that EU DXRGR EU MCTN EU EU MDS gene encoded protein was located in chloroplast EU ACOT and EU HMGR gene coding protein located in endoplasmic reticulum EU HMGS gene coding protein located on cytoplasmic membrane. The analysis of the spatiotemporal expression characteristics of the above genes by transcriptome sequencing showed that the genes related to the MEP pathway were highly expressed in the leaves of Eucommia ulmoides, while the MVA pathway genes were expressed in the young fruit of Eucommia ulmoides, and the rubber content in the young fruit of Eucommia ulmoides was higher than that in the leaves. Therefore, it is inferred that MVA pathway plays a leading role in the synthesis of Eucommia ulmoides.
【作者單位】： 中國(guó)林業(yè)科學(xué)研究院經(jīng)濟(jì)林研究開(kāi)發(fā)中心;國(guó)家林業(yè)局杜仲工程技術(shù)研究中心;
【基金】：國(guó)家十二五科技支撐計(jì)劃(2012BAD21B0502) 國(guó)家公益性行業(yè)科研專(zhuān)項(xiàng)(201004029)~~
【分類(lèi)號(hào)】：Q943.2;S567.19
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本文編號(hào)：2054416