本文選題：美容杜鵑 + RcHsfB��； 參考：《四川大學(xué)學(xué)報(自然科學(xué)版)》2017年02期

【摘要】：通過RT-PCR和RACE方法從美容杜鵑(Rhododendron calophytum)中克隆到一個熱激轉(zhuǎn)錄因子(Heat shock factors,Hsfs),命名為RcHsfB3(GenBank登錄號KU145694).RcHsfB3基因全長1130bp,完整的開放讀碼框(ORF)長771bp,預(yù)測可編碼257個氨基酸,分子量為28.8KD,等電點(diǎn)為5.38.系統(tǒng)進(jìn)化樹分析發(fā)現(xiàn),該基因與其它幾種植物的HsfB3基因?qū)儆谕环种?氨基酸同源序列比對結(jié)果表明RcHsfB3與葡萄的VvHsfB3的同源性最高,達(dá)67.29%.原生質(zhì)體亞細(xì)胞定位表明,RcHsfB3編碼蛋白定位在細(xì)胞核上.熒光定量PCR分析顯示,美容杜鵑分別受到熱激、低溫和鹽害脅迫處理后,RcHsfB3的相對表達(dá)量總體呈現(xiàn)先上升,后下降,且"上升-下降"交替的表達(dá)趨勢.初步研究表明RcHsfB3基因受高溫、低溫和高鹽脅迫的誘導(dǎo)表達(dá).
[Abstract]:A heat shock transcription factor (Heat shock factors) was cloned from Rhododendron calophytum by RT-PCR and race, named RcHsfB3 GenBank accession No. KU145694.RcHsfB3, the length of ORF was 771bp. it was predicted to encode 257 amino acids, the molecular weight was 28.8KDand the isoelectric point was 5.38bp. the total length of RcHsfB3 gene was 1130bp. the length of ORF was 771bp. the total length of RcHsfB3 gene was 771 bp. it was predicted to encode 257 amino acids with molecular weight of 28.8KDand isoelectric point of 5.38bp. Phylogenetic tree analysis showed that the HsfB3 gene belonged to the same branch as other plants. The results of amino acid sequence alignment showed that RcHsfB3 had the highest homology with VvHsfB3 of grape, reaching 67.29. The subcellular localization of protoplasts showed that RcHsfB3 encoded protein was located in the nucleus. Fluorescence quantitative PCR analysis showed that the relative expression of RcHsfB3 was increased first, then decreased, and the expression of RcHsfB3 was alternately increased and decreased under low temperature and salt stress, respectively. Preliminary studies showed that RcHsfB3 gene was induced to express under high temperature, low temperature and high salt stress.
【作者單位】： 四川大學(xué)生命科學(xué)學(xué)院;
【基金】：四川省科技廳應(yīng)用基礎(chǔ)項目(2012JY0090) 四川省科技基礎(chǔ)條件平臺項目—四川大學(xué)植物標(biāo)本數(shù)字化平臺建設(shè)及共享(2014JCp004)
【分類號】：Q943.2;S685.21

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